Human UNG antibody | NKMAX AUN0713 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human UNG antibody

catalog :

AUN0713

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

k1C12

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human UNG antibody

Catalog number (SKU) :

AUN0713

NCBI :

NP_550433

Description :

The human UNG gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms of uracil-DNA glycosylase (UNG). These forms are generated from transcription from alternative promoters, promoter A and promoter B respectively, and the subsequent use of alternative splicing. UNG is responsible for the removal of uracil from DNA by hydrolysis of the N-glycosidic bond that links the base to the deoxyribose backbone, leaving an abasic site. UNG is a highly conserved enzyme found in many species.

Alternative Names :

uracil-DNA glycosylase isoform UNG2, uracil-DNA glycosylase, UDG, UNG1, UNG2, HIGM4, uracil-DNA glycosylase 1, uracil-DNA glycosylase 2

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Jau der C et al.,(1991) J Bacteriol 173(1):283-290.;;Haug T et al.,(1998) Nucleic Acids Res. 26(6):1449-1457.;;Hilde N et al.,(2000) Nucleic Acids Res. 28(12):2277-2285.;;Stuart R.W. Bellamy et al.,(2007) Nucleic Acids Res. 35(5):1478-1487.;;Chih chung L et al.,(2007) J Virol 81(3):1195-1208.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human UNG (1-313aa) purified from E. coli

Applications :

ELISA,WB,ICC/IF

IF Comment And Pic :

ICC/IF analysis of UNG in HeLa cells. The cell was stained with AUN0713 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UNG antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: Jurkat cell lysate;;Lane 3.: MCF7 cell lysate;; Lane 4.: K562 cell lysate;; Lane 5.: 293T cell lysate;; Lane 6.: HepG2 cell lysate;; Lane 7.: A549 cell lysate;; Lane 8.: SK-OV-3 cell lysate;; Lane 9.: PC3 cell lysate

Reacts With :

Human

Clone Number :

k1C12

Ab Usage :

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Hormones & Cytokines

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P13051

Internal Search Terms :

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more info or order :

NKMAX product webpage