Human UbcH8/UBE2L6 antibody | NKMAX AUB0907 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human UbcH8/UBE2L6 antibody

catalog :

AUB0907

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

k1H3

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human UbcH8/UBE2L6 antibody

Catalog number (SKU) :

AUB0907

NCBI :

NP_004214

Description :

Ubiquitin-conjugating enzyme E2L6 (UBE2L6), also known as UbcH8, is a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination of a protein substrate requires the concerted action of 3 classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin protein ligases. The E2 ubiquitin-conjugating enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene.

Alternative Names :

Ubiquitin conjugating enzyme E2 L6, Ubiquitin/ISG15-conjugating enzyme E2 L6, E2 ubiquitin-conjugating enzyme L6, Retinoic acid-induced gene B protein, RIG-B, UbcH8, Ubiquitin carrier protein L6, Ubiquitin-protein ligase L6

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ardley HC., et al. (2000) Cytogenet Cell Genet. 89(1-2):137-140.;; Movnihan TP., et al. (1999) J Biol Chem. 274(43):30963-30968.;; Kumar S., et al. (1997) J Biol Chem. 272(21):13548-13554.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human UBE2L6 (1-152aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, IHC

IF Comment And Pic :

ICC/IF analysis ofUBE2L6 in HeLa cells. The cell was stained with AUB0907 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysate of MCF7 (20ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human UBE2L6 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;; Lane 1.: 293T cell lysate ;;Lane 2.: UBE2L6 Transfected 293T cell lysate ;;{AUB0907-WB2.jpg} The cell lysate (20ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ;;Lane 1.: Jurkat cell lysate ;;{AUB0907-WB3.jpg}

Reacts With :

Human

Clone Number :

k1H3

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

IHC Comment And Pic :

Paraffin embedded sections of human breast lobule tissue were incubated with anti-human UBE2L6 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Antigen Species :

Human

Gene Category :

Ubiquitination

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

O14933

Internal Search Terms :

UbcH8, UBE2L6,AUB0907-10ug,AUB0907-20ug,AUB0907-50ug,AUB0907-100ug,AUB0907-250ug,AUB0907-500ug,AUB0907-1mg,AUB0907-10,AUB0907-20,AUB0907-50,AUB0907-100,AUB0907-250,AUB0907-500,AUB0907-1

more info or order :

NKMAX product webpage