Human TREM2 antibody | NKMAX ATR0917 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human TREM2 antibody

catalog :

ATR0917

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2B5

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human TREM2 antibody

Catalog number (SKU) :

ATR0917

NCBI :

NP_061838

Description :

The triggering receptor expressed on myeloid cells-2 (TREM2) is a membrane protein that interacts with the TYROBP protein. The TREM2 and TYROBP proteins form a complex that transmits chemical signals to activate the cell. This complex is involved in the growth and development of several types of immune cells, particularly dendritic cells and activates these cells, triggering an inflammatory response to injury or disease. And the TYROBP-TREM2 complex also activates cells in the skeletal system and in the brain and spinal cord (central nervous system).

Alternative Names :

Ttriggering receptor expressed on myeloid cells 2 isoform 1, Triggering receptor expressed on myeloid cells 2, Triggering receptor expressed on myeloid cells 2a, TREM-2, Trem2a, Trem2b, Trem2c

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

N' Diaye EN, et al., (2009) J Cell Biol. 184(2):215-23.;; Piccio L, et al., (2008) Brain. 131(11):3081-91.

Abs Host :

Mouse

Isotype :

IgG3 kappa

Immunogen :

Recombinant human TREM2 (19-161aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of TREM2 in HepG2 cells. The cell was stained with ATR0917 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of TREM2 in Raw264.7 cells. The cell was stained with ATR0917 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATR0917-IF2.jpg}

WB Comment and Pic :

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TREM-2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1 : 293T cell lysate;; Lane 2 : TREM2 19-161aa Transfected 293T cell lysate;;

Reacts With :

Human

Clone Number :

2B5

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of TREM2 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

Antigen Species :

Human

Gene Category :

Immunology

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

Q9NZC2

Internal Search Terms :

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more info or order :

NKMAX product webpage