Recombinant human Flt3 ligand/FLT3LG protein | NKMAX ATGP4161 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Flt3 ligand/FLT3LG protein

catalog :

ATGP4161

quantity :

10 ug

price :

150 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Flt3 ligand/FLT3LG protein

Domain :

27-184aa

Catalog number (SKU) :

ATGP4161

NCBI :

NP_001450

Description :

FLT3LG, also known as fms-related tyrosine kinase 3 ligand, is an alpha-cytokine that promotes the differentiation of multiple hematopoietic cell lineages. This protein acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It is crucial for steady-state pDC and cDC development. It controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Additionally, FLT3LG synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. It cooperates with IL-2, IL-6, IL-7, and IL-15 to induce NK cell development and with IL-3, IL-7, and IL-11 to induce terminal B cell maturation. Animal studies show that it reduces the severity of experimentally induced allergic inflammation. Recombinant human FLT3LG, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Fms-related tyrosine kinase 3 ligand isoform 1, Fms-related tyrosine kinase 3 ligand, FLT3LG, FL, FLG3L, FLT3L, IMD125, Flt3 ligand, SL cytokine

Concentration :

0.5mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Klein O., et al. (2013) Eur. J. Immunol. 43(2):533-539.;; Nakamori Y., et al. (2012) Br. J. Haematol. 157(6):674-686.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cell proliferation assay using OCI-AML5 acute myeloid leukemia cells. The ED50 range ≤ 5 ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Angiogenesis

Additional Comment Only :

ATGP3272 has been replaced with a catalog number ATGP4161.

Molecular Weight :

19kDa (166aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human Flt3 ligand stimulates cell proliferation of the OCI-AML5 acute myeloid leukemia cells. The ED50 range ≤ 5 ng/ml.

Amino Acid Sequence :

TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTAPQP

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Thr 27

Product Type Name :

Recombinant Protein

UniProt No. :

P49771

Internal Search Terms :

ATGP4161-10ug, ATGP4161-20ug, ATGP4161-50ug, ATGP4161-100ug, ATGP4161-250ug, ATGP4161-500ug, ATGP4161-1mg, ATGP4161-010, ATGP4161-020, ATGP4161-050, ATGP4161-100, ATGP4161-250, ATGP4161-500, ATGP4161-01M, ATGP3272-10ug, ATGP3272-20ug, ATGP3272-50ug, ATGP3272-100ug, ATGP3272-250ug, ATGP3272-500ug, ATGP3272-1mg, ATGP3272-010, ATGP3272-020, ATGP3272-050, ATGP3272-100, ATGP3272-250, ATGP3272-500, ATGP3272-01M

more info or order :

NKMAX product webpage