Recombinant human TRANCE/RANK L/TNFSF11 protein | NKMAX ATGP4157 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human TRANCE/RANK L/TNFSF11 protein

catalog :

ATGP4157

quantity :

10 ug

price :

150 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human TRANCE/RANK L/TNFSF11 protein

Domain :

140-317aa

Catalog number (SKU) :

ATGP4157

NCBI :

NP_003692.1

Description :

TNFSF11, also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. TNFSF11 also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. TNFSF11 is important in bone metabolism. This natural and necessary surface-bound molecule (also known as CD254) found on osteoblasts serves to activate osteoclasts, which are the cells involved in bone resorption. Recombinant human TNFSF11 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Storage :

Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles

General References :

Lam J., et al. (2001) J. Clin. Invest. 108:971-979 Ito S., et al. (2002) J. Biol. Chem. 277:6631-6636

Purity :

> 85% by SDS – PAGE

Bioactivity Comment Only :

Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 range ≤ 25 ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Hormones & Cytokines

Molecular Weight :

22.3 kDa (199aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human TRANCE stimulates osteoclast differentiation in the RAW 264.7 mouse monocyte/macrophage cells. The ED50 range ≤ 25 ng/ml.

Amino Acid Sequence :

IRAEKAMVD GSWLDLAKRS KLEAQPFAHL TINATDIPSG SHKVSLSSWY HDRGWAKISN MTFSNGKLIV NQDGFYYLYA NICFRHHETS GDLATEYLQL MVYVTKTSIK IPSSHTLMKG GSTKYWSGNS EFHFYSINVG GFFKLRSGEE ISIEVSNPSL LDPDQDATYF GAFKVRDID

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Proteins

UniProt No. :

O14788

Internal Search Terms :

TRANCE , TNFSF11, RANK L, Tumor necrosis factor ligand superfamily member 11, OPGL, RANKL, ATGP4157-10ug,ATGP4157-20ug,ATGP4157-50ug,ATGP4157-100ug,ATGP4157-250ug,ATGP4157-500ug,ATGP4157-1mg,ATGP4157-10,ATGP4157-20,ATGP4157-50,ATGP4157-100,ATGP4157-250,ATGP4157-500,ATGP4157-1

more info or order :

NKMAX product webpage