protein

Recombinant human GFR alpha-1 protein

ATGP4154

10 ug

88 USD

Recombinant human GFR alpha-1 protein

25-424aa

ATGP4154

NP_665736.1

GFR alpha-1, also known as GDNF family receptor alpha-1, belongs to the GDNFR family. It is a glycosylphosphatidylinositol (GPI)-linked receptor found on the cell surface, acting as a receptor for both GDNF and NTN and function as neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. It bind GDNF strongly and activate RET tyrosine kinase and is also essential for the development of kidneys and the enteric nervous system. Recombinant human GFR alpha-1, fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

GDNF family receptor alpha-1 isoform b, GDNF receptor alpha-1, GDNF R alpha-1, GFR alpha1, GFR-ALPHA-1, GFRa-1, GFRalpha-1, GFRA1, GDNFR, GDNFRA, RET1L, RETL1, TRNR1, RHDA4, RET ligand, TGF-beta-related neurotrophic factor receptor 1

0.5 mg/ml (determined by absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117-126.;;Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801-5806.;;Jing S, et al. (1996). Cell. 85:1113-1124.;;Treanor JJ, et al. (1996). 382: 80-83.;;Jing S, et al. (1997). J Biol Chem. 272: 33111-33117.;;

> 90% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human GDNF (CAT# ATGP3942). The ED50 range ≤ 50 ng/ml.

SDS-PAGE, Bioactivity

Human

Neuroscience

70.8 kDa (633aa)

< 1 EU per 1ug of protein (determined by LAL method)

Human GDNF (CAT# ATGP3942) is coated at 0.5 ug/ml (100 ul/well) can bind Human GFR alpha-1. The ED50 range ≤ 50 ng/ml.

DRLDCVKASD QCLKEQSCST KYRTLRQCVA GKETNFSLAS GLEAKDECRS AMEALKQKSL YNCRCKRGMK KEKNCLRIYW SMYQSLQGND LLEDSPYEPV NSRLSDIFRV VPFISVEHIP KGNNCLDAAK ACNLDDICKK YRSAYITPCT TSVSNDVCNR RKCHKALRQF FDKVPAKHSY GMLFCSCRDI ACTERRRQTI VPVCSYEERE KPNCLNLQDS CKTNYICRSR LADFFTNCQP ESRSVSSCLK ENYADCLLAY SGLIGTVMTP NYIDSSSLSV APWCDCSNSG NDLEECLKFL NFFKDNTCLK NAIQAFGNGS DVTVWQPAFP VQTTTATTTT ALRVKNKPLG PAGSENEIPT HVLPPCANLQ AQKLKSNVSG NTHLCISNGN YEKEGLGASS

hIgG-Tag

HEK293

Asp 25

Recombinant Protein

P56159

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