Recombinant Animal Free Human EGF protein | NKMAX ATGP4153 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant Animal Free Human EGF protein

catalog :

ATGP4153

quantity :

10 ug

price :

48 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant Animal Free Human EGF protein

Domain :

971-1023aa

Catalog number (SKU) :

ATGP4153

NCBI :

NP_001954.2

Description :

Recombinant human epidermal growth factor (EGF) is a 6. 3 kDa globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.Produced using non-animal reagents in an animal-free laboratory

Alternative Names :

Epidermal growth factor, Pro-epidermal growth factor, urogastrone, EGF

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Riese., et al (1998 ) Bioessays. 20: 41-48.;; Cohen S., (1983) Cancer. 15: 1787-1791. ;; Carpenter G., et al (1979) Annu. Rev. Biochem., 48: 193-216.;;

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 0.1 ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Hormones & Cytokines

Molecular Weight :

6.3 kDa (54aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human EGF stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 0.1 ng/ml.

Amino Acid Sequence :

MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Animal Free Protein

UniProt No. :

P01133

Internal Search Terms :

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more info or order :

NKMAX product webpage