protein

Recombinant human Trypsin 3/PRSS3 protein

ATGP4130

10 ug

130 USD

Recombinant human Trypsin 3/PRSS3 protein

16-247aa

ATGP4130

NP_002762.2

PRSS3, also known as trypsin-3, is a member of the trypsin family of serine proteases. It is specialized for the degradation of trypsin inhibitors and may be involved in defensin processing, including DEFA5. This protein is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Compared to PRSS1 and 2, one intriguing feature of PRSS3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. It has been proposed to be degradation of trypsin inhibitors, which facilitates the digestion of those foods rich in these proteins. Recombinant human Trypsin 3/PRSS3, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Trypsin-3 isoform 2, Trypsin-3, PRSS3, MTG, PRSS4, T9, TRY3, TRY4, Brain trypsinogen, Mesotrypsin, Mesotrypsinogen, Serine protease 3, Serine protease 4

1mg/ml (determined by Absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Jiang G., et al. (2010) Gut 59:1535-1544.;; Rosendahl J., et al. (2010) Pancreatology 10:243-249.

> 95% by SDS-PAGE

Specific activity is > 10,000pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-RPKPVE-Nval-WRK(Dnp)-NH2 per minute at pH 8.0 at 37℃.

SDS-PAGE, Enzyme Activity

Human

Enzymes & Proteases, Ubiquitination, Cell Cycle

26kDa (238aa)

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 10,000pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-RPKPVE-Nval-WRK(Dnp)-NH2 per minute at pH 8.0 at 37℃.

VPFDDDDKIV GGYTCEENSL PYQVSLNSGS HFCGGSLISE QWVVSAAHCY KTRIQVRLGE HNIKVLEGNE QFINAAKIIR HPKYNRDTLD NDIMLIKLSS PAVINARVST ISLPTAPPAA GTECLISGWG NTLSFGADYP DELKCLDAPV LTQAECKASY PGKITNSMFC VGFLEGGKDS CQRDSGGPVV CNGQLQGVVS WGHGCAWKNR PGVYTKVYNY VDWIKDTIAA NS

His-Tag

HEK293

Val 16

Recombinant Protein

P35030

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