Recombinant human Insulysin/IDE protein | NKMAX ATGP4128 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Insulysin/IDE protein

catalog :

ATGP4128

quantity :

10 ug

price :

150 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Insulysin/IDE protein

Domain :

42-1019aa

Catalog number (SKU) :

ATGP4128

NCBI :

NP_004960.2

Description :

Insulysin/IDE, also known insulin-degrading enzyme, is a zinc metallopeptidase of the inverzincin family. This enzyme catalyses the degradation reaction of insulin, glucagon and other polypeptides. It is present in mammals and in many arthropods such as the fly Drosophila melanogaster. It is also expressed in many tissues, with the highest levels in liver, kidney, brain, and testis. It has been shown to degrade the amyloid beta peptide, which polymerizes into the plaques associated with Alzheimers disease. Therefore, the deficiency of IDE activity may contribute to the pathogenesis of type 2 diabetes mellitus and Alzheimers disease. Recombinant human Insulysin/IDE, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Insulin-degrading enzyme, insulin-degrading enzyme isoform 1, Abeta-degrading protease, Insulin protease, Insulysin, IDE

Concentration :

0.5mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 100mM NaCl, 0.05% Brij35, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Affholter J. A., et al. (1988) Science. 242:1415-1418.;; Duckworth W.C., et al. (1998) Endocr. Rev. 19:608-624.;; Akiyama H., et al. (1990) Biochem. Biophys. Res. Commun. 170:1325-1330.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 3,000 pmol/min/ug in which one unit will convert 1.0 pmole of Mca-RPPGFSAFK(Dnp)-OH to MCA-Pro-Leu-OH per minute at pH 7.5 at 25°C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Enzymes & Proteases, Neuroscience

Molecular Weight :

114kDa (984aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 3,000 pmol/min/ug in which one unit will convert 1.0 pmole of Mca-RPPGFSAFK(Dnp)-OH to MCA-Pro-Leu-OH per minute at pH 7.5 at 25°C.

Amino Acid Sequence :

MNNPAIKRIG NHITKSPEDK REYRGLELAN GIKVLLISDP TTDKSSAALD VHIGSLSDPP NIAGLSHFCE HMLFLGTKKY PKENEYSQFL SEHAGSSNAF TSGEHTNYYF DVSHEHLEGA LDRFAQFFLC PLFDESCKDR EVNAVDSEHE KNVMNDAWRL FQLEKATGNP KHPFSKFGTG NKYTLETRPN QEGIDVRQEL LKFHSAYYSS NLMAVCVLGR ESLDDLTNLV VKLFSEVENK NVPLPEFPEH PFQEEHLKQL YKIVPIKDIR NLYVTFPIPD LQKYYKSNPG HYLGHLIGHE GPGSLLSELK SKGWVNTLVG GQKEGARGFM FFIINVDLTE EGLLHVEDII LHMFQYIQKL RAEGPQEWVF QECKDLNAVA FRFKDKERPR GYTSKIAGIL HYYPLEEVLT AEYLLEEFRP DLIEMVLDKL RPENVRVAIV SKSFEGKTDR TEEWYGTQYK QEAIPDEVIK KWQNADLNGK FKLPTKNEFI PTNFEILPLE KEATPYPALI KDTAMSKLWF KQDDKFFLPK ACLNFEFFSP FAYVDPLHCN MAYLYLELLK DSLNEYAYAA ELAGLSYDLQ NTIYGMYLSV KGYNDKQPIL LKKIIEKMAT FEIDEKRFEI IKEAYMRSLN NFRAEQPHQH AMYYLRLLMT EVAWTKDELK EALDDVTLPR LKAFIPQLLS RLHIEALLHG NITKQAALGI MQMVEDTLIE HAHTKPLLPS QLVRYREVQL PDRGWFVYQQ RNEVHNNCGI EIYYQTDMQS TSENMFLELF CQIISEPCFN TLRTKEQLGY IVFSGPRRAN GIQGLRFIIQ SEKPPHYLES RVEAFLITME KSIEDMTEEA FQKHIQALAI RRLDKPKKLS AECAKYWGEI ISQQYNFDRD NTEVAYLKTL TKEDIIKFYK EMLAVDAPRR HKVSVHVLAR EMDSCPVVGE FPCQNDINLS QAPALPQPEV IQNMTEFKRG LPLFPLVKPH INFMAAKL

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Met 42

Product Type Name :

Recombinant Protein

UniProt No. :

P14735

Internal Search Terms :

ATGP4128-10ug, ATGP4128-20ug, ATGP4128-50ug, ATGP4128-100ug, ATGP4128-250ug, ATGP4128-500ug, ATGP4128-1mg, ATGP4128-010, ATGP4128-020, ATGP4128-050, ATGP4128-100, ATGP4128-250, ATGP4128-500, ATGP4128-01M

more info or order :

NKMAX product webpage