Recombinant human Alkaline Phosphatase/ALPL protein | NKMAX ATGP4125 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Alkaline Phosphatase/ALPL protein

catalog :

ATGP4125

quantity :

10 ug

price :

160 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Alkaline Phosphatase/ALPL protein

Domain :

18-501aa

Catalog number (SKU) :

ATGP4125

NCBI :

NP_000469.3

Description :

ALPL, also known as alkaline phosphatase, tissue-nonspecific isozyme, is one of alkaline phosphatases. Alkaline phosphatase is metabolizes various phosphate compounds and plays a key role in skeletal mineralization and adaptive thermogenesis. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue-nonspecific). Expression of this protein is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. It plays an essential role in skeletal and dental mineralization via its ability to hydrolyze extracellular diphosphate, a potent mineralization inhibitor, to phosphate. It thereby promotes hydroxyapatite crystal formation and increases inorganic phosphate concentration. Recombinant human Alkaline Phosphatase/ALPL, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names :

Aalkaline phosphatase, tissue-nonspecific isozyme isoform 1, Alkaline phosphatase liver/bone/kidney isozyme, Phosphoamidase, Phosphocreatine phosphatase, ALPL, AP-TNAP, APTNAP, HOPS, HPPA, HPPC, HPPI, HPPO, TNALP, TNAP, TNS-ALP, TNSALP

Concentration :

1mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Millan, J.L. and W.H. Fishman (1995) Crit. Rev. Clin. Lab. Sci. 32:1-39.;; Sara Sultana., et al, (2013) Mol Genet Metab. 109:282-288.;; Sonia Di Mauro., et al, (2002) J Bone Miner Res. 17:1383-1391.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 40,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Enzymes & Proteases, Cell Cycle

Molecular Weight :

54.3kDa (493aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Amino Acid Sequence :

LVPEKEK DPKYWRDQAQ ETLKYALELQ KLNTNVAKNV IMFLGDGMGV STVTAARILK GQLHHNPGEE TRLEMDKFPF VALSKTYNTN AQVPDSAGTA TAYLCGVKAN EGTVGVSAAT ERSRCNTTQG NEVTSILRWA KDAGKSVGIV TTTRVNHATP SAAYAHSADR DWYSDNEMPP EALSQGCKDI AYQLMHNIRD IDVIMGGGRK YMYPKNKTDV EYESDEKARG TRLDGLDLVD TWKSFKPRYK HSHFIWNRTE LLTLDPHNVD YLLGLFEPGD MQYELNRNNV TDPSLSEMVV VAIQILRKNP KGFFLLVEGG RIDHGHHEGK AKQALHEAVE MDRAIGQAGS LTSSEDTLTV VTADHSHVFT FGGYTPRGNS IFGLAPMLSD TDKKPFTAIL YGNGPGYKVV GGERENVSMV DYAHNNYQAQ SAVPLRHETH GGEDVAVFSK GPMAHLLHGV HEQNYVPHVM AYAACIGANL GHCAPAS

Tag :

His-Tag

Expression System :

HEK293

Predicted nterm :

Asp

Product Type Name :

Recombinant Protein

UniProt No. :

P05186

Internal Search Terms :

ATGP4125-10ug, ATGP4125-20ug, ATGP4125-50ug, ATGP4125-100ug, ATGP4125-250ug, ATGP4125-500ug, ATGP4125-1mg, ATGP4125-010, ATGP4125-020, ATGP4125-050, ATGP4125-100, ATGP4125-250, ATGP4125-500, ATGP4125-01M

more info or order :

NKMAX product webpage