protein

Recombinant Human Fc epsilon RI alpha protein

ATGP4124

10 ug

120 USD

Recombinant Human Fc epsilon RI alpha protein

26-205aa

ATGP4124

NP_001374209.1

Fc epsilon RI alpha, also known as FCER1A, is an IgE‑binding protein of the multichain immune recognition family. Fc epsilon receptor is a tetrameric complex of one alpha, one beta and two gamma subunits on mast cells and basophils. This protein represents the alpha subunit, only the alpha subunit is glycosylated. The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes—potent effectors of the hypersensitivity response. Recombinant human Fc epsilon RI alpha/FCER1A, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

High affinity immunoglobulin epsilon receptor subunit alpha isoform1, FCERIA, FCERA, Fc epsilon receptor Ia, Fc-epsilon RI-alpha, Fc epsilon RI alpha chain, FcERI, IgE Fc receptor subunit alpha, FCER1A, FCE1A

1mg/ml (determined by Absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

ZHANG,Y., et al. (2012) Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 47 (4), 289-293.;; Zhou,J., et al. (2012) Hum. Immunol. 73 (3), 301-305.;; D Maurer., et al. (1996) J Immunol. 157(2),607-616.

> 90% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human IgE. The ED50 range ≤ 0.01 ug/ml.

SDS-PAGE, Bioactivity

Human

Immunology

21.8kDa (186aa)

< 1 EU per 1ug of protein (determined by LAL method)

Human IgE is coated at 1 ug/ml (100 ul/well) can bind Human Fc epsilon RI alpha. The ED50 range ≤ 0.01 ug/ml.

VPQKPKVSLN PPWNRIFKGE NVTLTCNGNN FFEVSSTKWF HNGSLSEETN SSLNIVNAKF EDSGEYKCQH QQVNESEPVY LEVFSDWLLL QASAEVVMEG QPLFLRCHGW RNWDVYKVIY YKDGEALKYW YENHNISITN ATVEDSGTYY CTGKVWQLDY ESEPLNITVI KAPREKYWLQ

His-Tag

HEK293

Val 26

Recombinant Protein

P12319

ATGP4124-10ug, ATGP4124-20ug, ATGP4124-50ug, ATGP4124-100ug, ATGP4124-250ug, ATGP4124-500ug, ATGP4124-1mg, ATGP4124-010, ATGP4124-020, ATGP4124-050, ATGP4124-100, ATGP4124-250, ATGP4124-500, ATGP4124-01M