Recombinant human DSPG3 protein | NKMAX ATGP4123 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human DSPG3 protein

catalog :

ATGP4123

quantity :

10 ug

price :

120 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human DSPG3 protein

Domain :

20-322aa

Catalog number (SKU) :

ATGP4123

NCBI :

NP_004941.2

Description :

DSPG3, also called epiphycan/EPYC or PG-Lb, is a class III subfamily member of the SLRP(small leucine-rich proteoglycans) family. DSPG3 is found predominantly in epiphyseal cartilage, although DSPG3 mRNA is also detected in mouse testis and human ligament and placenta. In mouse, expression begins in mid-gestation as an intermediate marker for chondrogenesis, peaks at birth and declines thereafter. Deletion of DSPG3 in mouse results in a mild postnatal phenotype that worsens synergistically when the co‑expressed SLRP biglycan is also deleted. The double-mutant phenotype includes premature osteoarthritis, indicating a role for DSPG3, in cooperation with other SLRPs, in stability of the collagen matrix and maintenance of joint integrity. Recombinant human DSPG3, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names :

Epiphycan, Dermatan sulfate proteoglycan 3, Proteoglycan-Lb, PG-Lb, Small chondroitin/dermatan sulfate proteoglycan, EPYC, DSPG3, PGLB, SLRR3B, epiphycan proteoglycan

Concentration :

0.5mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Michelle Deere., et al, (1999) Genome Res. 9:449-456.;; Jan Johnson., et al, (1999) Dev. Dyn. 216:499-510.;; S. Nuka., et al, (2010) Osteoarthritis Cartilage 18:88-96.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured by the ability of the immobilized protein to support the adhesion of Saos‑2 human osteosarcoma cells. When cells are added to human DSPG3 1.25 ug/ml and human fibronectin 0.5 ug/ml coated plates. This effect is more to 60%.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Signal Transduction, Adhesion & Movement

Molecular Weight :

35.5kDa (309aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human DSPG3 and human fibronectin stimulates cell adhesion of the Saos‑2 human osteosarcoma cells. This effect is more to 60%.

Amino Acid Sequence :

APTLESINYD SETYDATLED LDNLYNYENI PVDKVEIEIA TVMPSGNREL LTPPPQPEKA QEEEEEEEST PRLIDGSSPQ EPEFTGVLGP HTNEDFPTCL LCTCISTTVY CDDHELDAIP PLPKNTAYFY SRFNRIKKIN KNDFASLSDL KRIDLTSNLI SEIDEDAFRK LPQLRELVLR DNKIRQLPEL PTTLTFIDIS NNRLGRKGIK QEAFKDMYDL HHLYLTDNNL DHIPLPLPEN LRALHLQNNN ILEMHEDTFC NVKNLTYIRK ALEDIRLDGN PINLSKTPQA YMCLPRLPVG SLV

Tag :

His-Tag

Expression System :

HEK293

Predicted nterm :

Ala 20

Product Type Name :

Recombinant Protein

UniProt No. :

Q99645

Internal Search Terms :

ATGP4123-10ug, ATGP4123-20ug, ATGP4123-50ug, ATGP4123-100ug, ATGP4123-250ug, ATGP4123-500ug, ATGP4123-1mg, ATGP4123-010, ATGP4123-020, ATGP4123-050, ATGP4123-100, ATGP4123-250, ATGP4123-500, ATGP4123-01M

more info or order :

NKMAX product webpage