protein

Recombinant human APP/Protease Nexin II protein

ATGP4122

10 ug

150 USD

Recombinant human APP/Protease Nexin II protein

18-701aa

ATGP4122

NP_000475.1

APP, also known as amyloid beta A4 protein, functions as a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. It has been implicated as a regulator of synapse formation, neural plasticity, antimicrobial activity, and iron export. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebral arterial amyloidosis. Recombinant human APP, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

ABPP, APPI, Alzheimer disease amyloid A4 protein homolog, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta precursor protein, Amyloid-beta A4 protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II, A4, AD1, beta-amyloid peptide, beta-amyloid precursor protein, testicular tissue protein Li 2, AAA, ABETA, alpha-sApp, CTF gamma, PN2

0.5mg/ml (determined by Absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Kontush A., et al. (2001) Cell. Mol. Neurobiol. 21:299-315.;; Walter M.F., et al. (2006) Biochem. Biophys. Res. Commun. 233:760-764.

> 90% by SDS-PAGE

The IC50 value is ≤ 1 nM, The inhibitory function of APP on activity of trypsin was measured by a fluorometric assay using Mca-RPKPVE-Nval-WRK(Dnp)-NH2 at pH 7.5 at 37C.

SDS-PAGE, Enzyme Activity

Human

Neuroscience

78.2kDa (690aa)

< 1 EU per 1ug of protein (determined by LAL method)

The IC50 value is ≤ 1 nM, The inhibitory function of APP on activity of trypsin was measured by a fluorometric assay using Mca-RPKPVE-Nval-WRK(Dnp)-NH2 at pH 7.5 at 37C.

LEVPTDGNAG LLAEPQIAMF CGRLNMHMNV QNGKWDSDPS GTKTCIDTKE GILQYCQEVY PELQITNVVE ANQPVTIQNW CKRGRKQCKT HPHFVIPYRC LVGEFVSDAL LVPDKCKFLH QERMDVCETH LHWHTVAKET CSEKSTNLHD YGMLLPCGID KFRGVEFVCC PLAEESDNVD SADAEEDDSD VWWGGADTDY ADGSEDKVVE VAEEEEVAEV EEEEADDDED DEDGDEVEEE AEEPYEEATE RTTSIATTTT TTTESVEEVV REVCSEQAET GPCRAMISRW YFDVTEGKCA PFFYGGCGGN RNNFDTEEYC MAVCGSAMSQ SLLKTTQEPL ARDPVKLPTT AASTPDAVDK YLETPGDENE HAHFQKAKER LEAKHRERMS QVMREWEEAE RQAKNLPKAD KKAVIQHFQE KVESLEQEAA NERQQLVETH MARVEAMLND RRRLALENYI TALQAVPPRP RHVFNMLKKY VRAEQKDRQH TLKHFEHVRM VDPKKAAQIR SQVMTHLRVI YERMNQSLSL LYNVPAVAEE IQDEVDELLQ KEQNYSDDVL ANMISEPRIS YGNDALMPSL TETKTTVELL PVNGEFSLDD LQPWHSFGAD SVPANTENEV EPVDARPAAD RGLTTRPGSG LTNIKTEEIS EVKMDAEFRH DSGYEVHHQK LVFFAEDVGS NKGA

His-Tag

HEK293

Leu 18

Recombinant Protein

P05067

ATGP4122-10ug, ATGP4122-20ug, ATGP4122-50ug, ATGP4122-100ug, ATGP4122-250ug, ATGP4122-500ug, ATGP4122-1mg, ATGP4122-010, ATGP4122-020, ATGP4122-050, ATGP4122-100, ATGP4122-250, ATGP4122-500, ATGP4122-01M