Recombinant human HAPLN1 protein | NKMAX ATGP4121 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human HAPLN1 protein

catalog :

ATGP4121

quantity :

10 ug

price :

160 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human HAPLN1 protein

Domain :

16-354aa

Catalog number (SKU) :

ATGP4121

NCBI :

NP_001875.1

Description :

HAPLN1, also known as CRTL1, is a member of the hyaladherin family. This protein contains a common structural domain of about 100 amino acids that is termed a Link module with two α-helices and two antiparallel β-sheets. It involved in the formation and stability of extracellular matrix via its association with specific HA-binding proteins. It stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix. Also, It has been shown to interact with Versican, two extracellular matrix components essential for cardiac development. Recombinant human HAPLN1, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Alternative Names :

hyaluronan and proteoglycan link protein 1, CRT1, CRTL1, HPLN1, Cartilage-linking protein 1, Proteoglycan link protein

Concentration :

0.25mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Zhang T., et al. (2022) Gastric Cancer. 25:346-359.;; Sun J., et al. (2022) Circulation. 146:48-63.;; Day, A.J. and G.D. Prestwich (2002) J. Biol. Chem. 277:4585-4588.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured by its binding ability in a functional ELISA with Hyaluronic acid. The ED50 range ≤ 1 ug/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Signal Transduction, Cytoskeleton

Molecular Weight :

39.3kDa (345aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Hyaluronic acid is coated at 10 ug/ml (100 ul/well) can bind Human HAPLN1. The ED50 range ≤ 1 ug/ml.

Amino Acid Sequence :

DHLSDNYTLD HDRAIHIQAE NGPHLLVEAE QAKVFSHRGG NVTLPCKFYR DPTAFGSGIH KIRIKWTKLT SDYLKEVDVF VSMGYHKKTY GGYQGRVFLK GGSDSDASLV ITDLTLEDYG RYKCEVIEGL EDDTVVVALD LQGVVFPYFP RLGRYNLNFH EAQQACLDQD AVIASFDQLY DAWRGGLDWC NAGWLSDGSV QYPITKPREP CGGQNTVPGV RNYGFWDKDK SRYDVFCFTS NFNGRFYYLI HPTKLTYDEA VQACLNDGAQ IAKVGQIFAA WKILGYDRCD AGWLADGSVR YPISRPRRRC SPTEAAVRFV GFPDKKHKLY GVYCFRAYN

Tag :

His-Tag

Expression System :

HEK293

Predicted nterm :

Asp

Product Type Name :

Recombinant Protein

UniProt No. :

P10915

Internal Search Terms :

ATGP4121-10ug, ATGP4121-20ug, ATGP4121-50ug, ATGP4121-100ug, ATGP4121-250ug, ATGP4121-500ug, ATGP4121-1mg, ATGP4121-010, ATGP4121-020, ATGP4121-050, ATGP4121-100, ATGP4121-250, ATGP4121-500, ATGP4121-01M

more info or order :

NKMAX product webpage