Recombinant mouse ICAM-1/CD54 protein | NKMAX ATGP4119 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse ICAM-1/CD54 protein

catalog :

ATGP4119

quantity :

10 ug

price :

94 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse ICAM-1/CD54 protein

Domain :

28-485aa

Catalog number (SKU) :

ATGP4119

NCBI :

NP_034623.1

Description :

ICAM-1, also known as CD54 (Cluster of Differentiation 54), belongs to the ICAM proteins. This protein is ligand for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 and then transmigrate into tissues. During leukocyte trans-endothelial migration, ICAM-1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. It is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. Also, It has been implicated in the progression of autoimmune diseases. Recombinant mouse ICAM1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

ICAM1, CD54, Icam-1, Ly-47, MALA-2, Intercellular adhesion molecule 1, MyD10

Concentration :

0.5mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Siu G., et al. (1989) J. Immunol. 143:3813-3820.;; Lord K. A., et al. (1990) Oncogene 5:387-396.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured by the ability of the immobilized protein to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM-1/CD54 coated plates 5ug/ml. This effect is more to 60%.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Mouse

Gene Category :

Immunology, Adhesion & Movement

Molecular Weight :

51.2 kDa (466aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Mouse ICAM-1/CD54 stimulates cell adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cells. This effect is more to 60%.

Amino Acid Sequence :

QVSIHPREAF LPQGGSVQVN CSSSCKEDLS LGLETQWLKD ELESGPNWKL FELSEIGEDS SPLCFENCGT VQSSASATIT VYSFPESVEL RPLPAWQQVG KDLTLRCHVD GGAPRTQLSA VLLRGEEILS RQPVGGHPKD PKEITFTVLA SRGDHGANFS CRTELDLRPQ GLALFSNVSE ARSLRTFDLP ATIPKLDTPD LLEVGTQQKL FCSLEGLFPA SEARIYLELG GQMPTQESTN SSDSVSATAL VEVTEEFDRT LPLRCVLELA DQILETQRTL TVYNFSAPVL TLSQLEVSEG SQVTVKCEAH SGSKVVLLSG VEPRPPTPQV QFTLNASSED HKRSFFCSAA LEVAGKFLFK NQTLELHVLY GPRLDETDCL GNWTWQEGSQ QTLKCQAWGN PSPKMTCRRK ADGALLPIGV VKSVKQEMNG TYVCHAFSSH GNVTRNVYLT VLYHSQNN

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Gln 28

Product Type Name :

Recombinant Protein

UniProt No. :

P13597

Internal Search Terms :

ATGP4119-10ug, ATGP4119-20ug, ATGP4119-50ug, ATGP4119-100ug, ATGP4119-250ug, ATGP4119-500ug, ATGP4119-1mg, ATGP4119-010, ATGP4119-020, ATGP4119-050, ATGP4119-100, ATGP4119-250, ATGP4119-500, ATGP4119-01M, ATGP3268-10ug, ATGP3268-20ug, ATGP3268-50ug, ATGP3268-100ug, ATGP3268-250ug, ATGP3268-500ug, ATGP3268-1mg, ATGP3268-010, ATGP3268-020, ATGP3268-050, ATGP3268-100, ATGP3268-250, ATGP3268-500, ATGP3268-01M

more info or order :

NKMAX product webpage