Recombinant human Fc gamma RIIA/CD32a (R167H) protein | NKMAX ATGP4116 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Fc gamma RIIA/CD32a (R167H) protein

catalog :

ATGP4116

quantity :

10 ug

price :

130 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Fc gamma RIIA/CD32a (R167H) protein

Domain :

30-218aa

Catalog number (SKU) :

ATGP4116

NCBI :

AAA35827.1

Description :

Fc gamma RIIA/CD32a, also known as Low affinity immunoglobulin gamma Fc region receptor II-a, are members of the Ig superfamily that function in the activation or inhibition of immune responses. Fc gamma RIIA is expressed on many immune cell types where inhibitory ITIM-bearing receptors may also be co-expressed and co-engaged by specific ligands. Signaling through Fc gamma RIIA results in the initiation of inflammatory responses that can be modulated by signals from the inhibitory receptors. The strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors. Besides IC, Fc gamma RII A also binds C-reactive protein (CRP) Two allelic variants of Fc gamma RIIA that differ in their ability to ligate human IgG2 or CRP exist. The R167H allele has been found to have a protective effect against lupus nephritis. Recombinant human Fc gamma RIIA/CD32a, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names :

Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, CDw32 Fc-gamma RII-a, Fc-gamma-RIIa, FcRII-a, CD32, FCGR2A, FCG2, FCGR2A1, IGFR2, CD32a, FCG2A

Concentration :

1mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275-290.;; Chi, M., et al. (2002) J. Immunol. 168:3697-3701.;; Zuniga. R., et al. (2003) Arthritis Rheum. 48:460.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured by its binding ability in a functional ELISA with Human IgG1 Fc (CAT# ATGP4094). The ED50 range ≤5 ug/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Immunology

Molecular Weight :

21.6kDa (195aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human IgG1 Fc (CAT# ATGP4094) is coated at 1 ug/ml (100 ul/well) can bind Human Fc gamma RIIA/CD32a (H167). The ED50 range ≤5 ug/ml.

Amino Acid Sequence :

SADSQAAAPP KAVLKLEPPW INVLQEDSVT LTCQGARSPE SDSIQWFHNG NLIPTHTQPS YRFKANNNDS GEYTCQTGQT SLSDPVHLTV LSEWLVLQTP HLEFQEGETI MLRCHSWKDK PLVKVTFFQN GKSQKFSHLD PTFSIPQANH SHSGDYHCTG NIGYTLFSSK PVTITVQVPS MGSSSPMGI

Tag :

His-Tag

Expression System :

HEK293

Predicted nterm :

Ser 30

Product Type Name :

Recombinant Protein

UniProt No. :

P12318

Internal Search Terms :

ATGP4116-10ug, ATGP4116-20ug, ATGP4116-50ug, ATGP4116-100ug, ATGP4116-250ug, ATGP4116-500ug, ATGP4116-1mg, ATGP4116-010, ATGP4116-020, ATGP4116-050, ATGP4116-100, ATGP4116-250, ATGP4116-500, ATGP4116-01M

more info or order :

NKMAX product webpage