protein

Recombinant human ICAM-1/CD54 protein

ATGP4113

10 ug

76 USD

Recombinant human ICAM-1/CD54 protein

28-480aa

ATGP4113

NP_000192.1

ICAM-1/CD54, also known as intercellular adhesion molecule 1, is a member of the immunoglobulin superfamily. It expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFNG, TNFa, IL-1b and LPS. They are important in inflammation, immune responses and in intracellular signalling events. It is known to bind to leucocyte integrins CD11/CD18 such as LFA-1 and Macrophage-1 antigen, during inflammation and in immune responses. It has been implicated in subarachnoid hemorrhage and expressed by respiratory epithelial cells is also the binding site for rhinovirus, the causative agent of most common colds. Recombinant human ICAM-1/CD54, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Intercellular adhesion molecule 1, ICAM1, Major group rhinovirus receptor, BB2, CD54

0.25mg/ml (determined by Bradford assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Yusuf-Makagiansar H. et al. (2001) Peptides. 22:1955-1962

> 90% by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of HL-60 human promyelocytic cells. When cells are added to Human ICAM-1/CD54 coated plates, the ED50 range ≤ 2 ug/ml.

SDS-PAGE, Bioactivity

Human

Immunology, Adhesion & Movement

ATGP3030 has been replaced with a catalog number ATGP4113.

76.5 kDa (692aa)

< 1 EU per 1ug of protein (determined by LAL method)

Human ICAM-1/CD54 stimulates cell adhesion of the HL-60 human promyelocytic cells. The ED50 range ≤ 2 ug/ml.

QTSVSPSKVI LPRGGSVLVT CSTSCDQPKL LGIETPLPKK ELLLPGNNRK VYELSNVQED SQPMCYSNCP DGQSTAKTFL TVYWTPERVE LAPLPSWQPV GKNLTLRCQV EGGAPRANLT VVLLRGEKEL KREPAVGEPA EVTTTVLVRR DHHGANFSCR TELDLRPQGL ELFENTSAPY QLQTFVLPAT PPQLVSPRVL EVDTQGTVVC SLDGLFPVSE AQVHLALGDQ RLNPTVTYGN DSFSAKASVS VTAEDEGTQR LTCAVILGNQ SQETLQTVTI YSFPAPNVIL TKPEVSEGTE VTVKCEAHPR AKVTLNGVPA QPLGPRAQLL LKATPEDNGR SFSCSATLEV AGQLIHKNQT RELRVLYGPR LDERDCPGNW TWPENSQQTP MCQAWGNPLP ELKCLKDGTF PLPIGESVTV TRDLEGTYLC RARSTQGEVT REVTVNVLSP RYE

hIgG-His-Tag

Baculovirus

Gln28

Recombinant Protein

P05362

ICAM1, CD54, Intercellular adhesion molecule 1, Intercellular adhesion molecule 1, BB2, CD54,ATGP4113-10ug,ATGP4113-20ug,ATGP4113-50ug,ATGP4113-100ug,ATGP4113-250ug,ATGP4113-500ug,ATGP4113-1mg,ATGP4113-10,ATGP4113-20,ATGP4113-50,ATGP4113-100,ATGP4113-250,ATGP4113-500,ATGP4113-1,ATGP3030-10ug, ATGP3030-20ug, ATGP3030-50ug, ATGP3030-100ug, ATGP3030-250ug, ATGP3030-500ug, ATGP3030-1mg, ATGP3030-010, ATGP3030-020, ATGP3030-050, ATGP3030-100, ATGP3030-250, ATGP3030-500, ATGP3030-01M