Recombinant mouse BACE-2 protein | NKMAX ATGP4110 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse BACE-2 protein

catalog :

ATGP4110

quantity :

10 ug

price :

160 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse BACE-2 protein

Domain :

20-462aa

Catalog number (SKU) :

ATGP4110

NCBI :

NP_062390.3

Description :

BACE-2, also known as beta secretase 2, belongs to the peptidase A1 family. It is an aspartic protease that is a close homolog of BACE-1. However, BACE-2 differs from BACE-1 in several aspects including pro-enzyme activation, substrate binding sites, transcriptional regulation, and function in Aβ peptide production. It has been shown to play a key role in insulin receptor trafficking in the pancreas where it is expressed in β cells and affects glucose tolerance and was suggested as a promising target for improving β cell function in diabetes. Recombinant mouse BACE-2, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names :

ARP1, ASP1, ASP21, BAE2, CDA13, CEAP1, DRAP, 1110059C24Rik, AEPLC, AI850424, ALP56, beta-site APP-cleaving enzyme 2, beta-secretase 2, Aspartyl protease 1, Asp 1, Beta-site amyloid precursor protein cleaving enzyme 2, Memapsin-1, Membrane-associated aspartic protease 1, Theta-secretase

Concentration :

0.25mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Sun, X. et al. (2006) FASEB J. 19:739-749.;; Hussain, I. et al. (2001) J. Biol. Chem. 276:23322-23328.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 20 pmol/min/ug in which one unit will convert 1.0pmole of Mca-SEVNLDAEFRK(Dnp)RR-NH2 to Mca- Pro-Leu-OH per minute at pH 3.5 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Mouse

Gene Category :

Protease inhibitors, Neuroscience

Molecular Weight :

48.6kDa (449aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 20 pmol/min/ug in which one unit will convert 1.0pmole of Mca-SEVNLDAEFRK(Dnp)RR-NH2 to Mca- Pro-Leu-OH per minute at pH 3.5 at 25C.

Amino Acid Sequence :

AVPALAPAPF TLPLQVARAT NHRASAVPGL GTPELPRADG LALALEPVRA TANFLAMVDN LQGDSGRGYY LEMLIGTPPQ KVQILVDTGS SNFAVAGAPH SYIDTYFDSE SSSTYHSKGF DVTVKYTQGS WTGFVGEDLV TIPKGFNSSF LVNIATIFES ENFFLPGIKW NGILGLAYAA LAKPSSSLET FFDSLVAQAK IPDIFSMQMC GAGLPVAGSG TNGGSLVLGG IEPSLYKGDI WYTPIKEEWY YQIEILKLEI GGQNLNLDCR EYNADKAIVD SGTTLLRLPQ KVFDAVVEAV ARTSLIPEFS DGFWTGAQLA CWTNSETPWA YFPKISIYLR DENASRSFRI TILPQLYIQP MMGAGFNYEC YRFGISSSTN ALVIGATVME GFYVVFDRAQ RRVGFAVSPC AEIEGTTVSE ISGPFSTEDI ASNCVPAQAL NEP

Tag :

His-Tag

Expression System :

HEK293

Predicted nterm :

Ala 20

Product Type Name :

Recombinant Protein

UniProt No. :

Q9JL18

Internal Search Terms :

ATGP4110-10ug,ATGP4110-20ug,ATGP4110-50ug,ATGP4110-100ug,ATGP4110-250ug,ATGP4110-500ug,ATGP4110-1mg,ATGP4110-10,ATGP4110-20,ATGP4110-50,ATGP4110-100,ATGP4110-250,ATGP4110-500,ATGP4110-1

more info or order :

NKMAX product webpage