protein

Recombinant human CD4 protein

ATGP4108

10 ug

120 USD

Recombinant human CD4 protein

26-390aa

ATGP4108

NP_000607.1

CD4, also known as T-cell surface antigen T4/Leu-3, is a member of the immunoglobulin superfamily. It is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. It interacts directly with MHC class II molecules on the surface of the antigen presenting cell via its extracellular domain. It is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen presenting cell. Also, it binds directly to MHC class II molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. Recombinant human CD4, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

T-cell surface glycoprotein CD4, CD4, CD4mut, T-cell surface glycoprotein CD4 isoform 1, T-cell surface antigen T4/Leu-3, OKT4D

0.25mg/ml (determined by Absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 40% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Singh SK., et al. (2012) FEBS J. 279:3705-3714.;; Farrar WL., et al. (1988) Crit Rev Immunol. 8:315-339.;; Doyle, C. and J.L. Strominger (1987) Nature 330:256-259.;;

90% by SDS - PAGE

Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. When cells are added to human CD4 coated plates. The ED50 range ≤ 10 ug/ml.

SDS-PAGE, Bioactivity

Human

Immunology

67.7kDa (604aa)

<1 EU per 1ug of protein (determined by LAL method)

Human CD4 stimulates cell adhesion of the HeLa human cervical epithelial carcinoma cells. The ED50 range ≤ 10 ug/ml.

KKVVLGKKGD TVELTCTASQ KKSIQFHWKN SNQIKILGNQ GSFLTKGPSK LNDRADSRRS LWDQGNFPLI IKNLKIEDSD TYICEVEDQK EEVQLLVFGL TANSDTHLLQ GQSLTLTLES PPGSSPSVQC RSPRGKNIQG GKTLSVSQLE LQDSGTWTCT VLQNQKKVEF KIDIVVLAFQ KASSIVYKKE GEQVEFSFPL AFTVEKLTGS GELWWQAERA SSSKSWITFD LKNKEVSVKR VTQDPKLQMG KKLPLHLTLP QALPQYAGSG NLTLALEAKT GKLHQEVNLV VMRATQLQKN LTCEVWGPTS PKLMLSLKLE NKEAKVSKRE KAVWVLNPEA GMWQCLLSDS GQVLLESNIK VLPTW

hIgG-His-Tag

HEK293

Lys26

Recombinant Protein

P01730

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