Recombinant rat MMP-9 protein | NKMAX ATGP4106 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant rat MMP-9 protein

catalog :

ATGP4106

quantity :

10 ug

price :

120 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant rat MMP-9 protein

Domain :

20-708aa

Catalog number (SKU) :

ATGP4106

NCBI :

NP_112317.1

Description :

MMP-9, also known matrix metalloproteinase-9, is one of the matrix metalloproteinases superfamily which is zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. It degrades many substrates such as gelatin, collagens, elastin and proteoglycan core protein which appears to be involved in invasive ability. This protein also plays an essential role in leukocyte migration and in bone osteoclastic resorption. It plays an important role in angiogenesis and neovascularization and so appears to be involved in the remodeling associated with malignant glioma neovascularization. Recombinant rat MMP-9 protein, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names :

Matrix metalloproteinase-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, Mmp9, MANDP2, AW743869, B/MMP, B/MMP9, Clg4, Clg4b, Gel B, MMP-9, pro-MMP-9

Concentration :

0.5mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid in. 20mM Tris-HCl (pH 7.5) containing 1mM CaCl2, 100mM NaCl, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Lee YD., et al, (2014) BMB Rep. 47:262-267.;; Matin S., et al, (2018) Int J Chron Obstruct Pulmon Dis. 13:1449-1454.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 2,000 pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-PLGL-Dpa-AR-NH2 per minute at pH 7.5 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Rat

Gene Category :

Angiogenesis, Cardiovascular, Adhesion & Movement

Molecular Weight :

77.2kDa (695aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 2,000 pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-PLGL-Dpa-AR-NH2 per minute at pH 7.5 at 25C.

Amino Acid Sequence :

APHQRQPTYV VFPRDLKTSN LTDTQLAEDY LYRYGYTRAA QMMGEKQSLR PALLMLQKQL SLPQTGELDS ETLKAIRSPR CGVPDVGKFQ TFEGDLKWHH HNITYWIQSY TEDLPRDVID DSFARAFAVW SAVTPLTFTR VYGLEADIVI QFGVAEHGDG YPFDGKDGLL AHAFPPGPGI QGDAHFDDDE LWSLGKGAVV PTYFGNANGA PCHFPFTFEG RSYLSCTTDG RNDGKPWCGT TADYDTDRKY GFCPSENLYT EHGNGDGKPC VFPFIFEGHS YSACTTKGRS DGYRWCATTA NYDQDKLYGF CPTRADVTVT GGNSAGEMCV FPFVFLGKQY STCTGEGRSD GRLWCATTSN FDADKKWGFC PDQGYSLFLV AAHEFGHALG LDHSSVPEAL MYPMYHYHED SPLHEDDIKG IQHLYGRGSK PDPRPPATTA AEPQPTAPPT MCPTAPPMAY PTGGPTVAPT GAPSPGPTGP PTAGPSEAPT ESSTPVDNPC NVDVFDAIAD IQGALHFFKD GRYWKFSNHG GSQLQGPFLI ARTWPALPAK LNSAFEDPQS KKIFFFSGRK MWVYTGQTVL GPRSLDKLGL GSEVTLVTGL LPRRGGKALL ISRERIWKFD LKSQKVDPQS VTRLDNEFSG VPWNSHNVFH YQDKAYFCHD KYFWRVSFHN RVNQVDHVAY VTYDLLQCP

Tag :

His-Tag

Expression System :

HEK293

Predicted nterm :

Ala 20

Product Type Name :

Recombinant Protein

UniProt No. :

P50282

Internal Search Terms :

ATGP4106-10ug,ATGP4106-20ug,ATGP4106-50ug,ATGP4106-100ug,ATGP4106-250ug,ATGP4106-500ug,ATGP4106-1mg,ATGP4106-10,ATGP4106-20,ATGP4106-50,ATGP4106-100,ATGP4106-250,ATGP4106-500,ATGP4106-1

more info or order :

NKMAX product webpage