Recombinant human IGFBP-1 protein | NKMAX ATGP4103 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human IGFBP-1 protein

catalog :

ATGP4103

quantity :

10 ug

price :

160 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human IGFBP-1 protein

Domain :

26-259aa

Catalog number (SKU) :

ATGP4103

NCBI :

NP_000587.1

Description :

IGFBP-1(Insulin-like growth factor-binding protein 1), also known as PP12(placental protein 12), is a member of the Insulin-like growth factor-binding protein family. This protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGFBP-1 is expressed in liver, decidua, kidneys and is the most abundant IGFBP in amniotic fluid. Serum levels of IGFBP-1 are lowest after meals. Hepatocyte production of IGFBP-1 is regulated at the transcriptional level due to the affects of insulin and corticosteriods. Recombinant human IGFBP-1 was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names :

insulin like growth factor binding protein 1, IGFBP1, AFBP, hIGFBP-1, IBP1, IGF-BP25, PP12, IGF-binding protein 1, Placental protein 12, amniotic fluid binding protein, alpha-pregnancy-associated endometrial globulin

Concentration :

0.5mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3-34.;; Kelley, K.M., et al, (1996) Int. J. Biochem. Cell Biol. 28:619-637.;; J.Ekstrand., et al, (1990) Genomics. 6:413-418.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured by its ability to inhibit proliferation using MCF-7 human breast cancer cells in the presence of Human IGF-1. The ED50 range ≤3 ug/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Signal Transduction

Molecular Weight :

25.2kDa (234aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human IGFBP-1 inhibit proliferation using MCF-7 human breast cancer cells in the presence of Human IGF-1. The ED50 range ≤3 ug/ml.

Amino Acid Sequence :

APWQCAPCSA EKLALCPPVS ASCSEVTRSA GCGCCPMCAL PLGAACGVAT ARCARGLSCR ALPGEQQPLH ALTRGQGACV QESDASAPHA AEAGSPESPE STEITEEELL DNFHLMAPSE EDHSILWDAI STYDGSKALH VTNIKKWKEP CRIELYRVVE SLAKAQETSG EEISKFYLPN CNKNGFYHSR QCETSMDGEA GLCWCVYPWN GKRIPGSPEI RGDPNCQIYF NVQN

Tag :

Non-Tagged

Expression System :

HEK293

Predicted nterm :

Ala26

Product Type Name :

Recombinant Protein

UniProt No. :

P08833

Internal Search Terms :

ATGP4103-10ug,ATGP4103-20ug,ATGP4103-50ug,ATGP4103-100ug,ATGP4103-250ug,ATGP4103-500ug,ATGP4103-1mg,ATGP4103-10,ATGP4103-20,ATGP4103-50,ATGP4103-100,ATGP4103-250,ATGP4103-500,ATGP4103-1

more info or order :

NKMAX product webpage