protein

Recombinant human VEGFR1/Flt-1 protein

ATGP4101

10 ug

158 USD

Recombinant human VEGFR1/Flt-1 protein

27-328aa

ATGP4101

NP_002010.2

VEGFR1(Vascular endothelial growth factor receptor 1), also known as FLT-1(Fms-like tyrosine kinase 1), is belongs to the class III subfamily of receptor tyrosine kinases. While family members VEGFR1, VEGFR2and VEGFR3 are all mainly expressed on endothelial cells, only VEGFR1 is expressed on macrophages, and mainly plays inhibitory roles. Inhibitors of VEGFR are used in the treatment of cancer. It acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. VEGFR1 binds VEGF with higher affinity than does VEGFR2, but shows weaker kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Recombinant human VEGFR1/Flt-1, fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

VEGFR1, VEGF R1, VEGFR-1, Vascular endothelial growth factor receptor 1 isoform 1, Fms-like tyrosine kinase 1, FLT-1, FLT1, FLT, FRT, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular permeability factor receptor, fms-related tyrosine kinase 1, vascular endothelial growth factor, vascularpermeability factor receptor

0.5mg/ml (determined by absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Peters, K.G. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8915-8919.;; Kendall R.L., et al., (1993), Natl. Acad. Sci. U.S.A. 90:10705-10709.;; Herley M.T., et al.,(1999), Biochem. Biophys. Res. Commun. 262:731-738.;; Kendall R.L., et al., (1993), Natl. Acad. Sci. U.S.A. 90:10705-10709.;;

> 90% by SDS-PAGE

Measured by its ability to inhibit proliferation using HUVEC human umbilical vein endothelial cells in the presence of Human VEGF165. The ED50 range ≤ 60ng/ml.

SDS-PAGE, Bioactivity

Human

Angiogenesis, Cardiovascular

60.3kDa (535aa)

< 1 EU per 1ug of protein (determined by LAL method)

Human VEGFR1/Flt-1 inhibit proliferation of the HUVEC human umbilical vein endothelial cells in the presence of Human VEGF165. The ED50 range ≤ 60 ng/ml.

SKLKDPELSL KGTQHIMQAG QTLHLQCRGE AAHKWSLPEM VSKESERLSI TKSACGRNGK QFCSTLTLNT AQANHTGFYS CKYLAVPTSK KKETESAIYI FISDTGRPFV EMYSEIPEII HMTEGRELVI PCRVTSPNIT VTLKKFPLDT LIPDGKRIIW DSRKGFIISN ATYKEIGLLT CEATVNGHLY KTNYLTHRQT NTIIDVQIST PRPVKLLRGH TLVLNCTATT PLNTRVQMTW SYPDEKNKRA SVRRRIDQSN SHANIFYSVL TIDKMQNKDK GLYTCRVRSG PSFKSVNTSV HI

hIgG-Tag

HEK293

Ser 27

Recombinant Protein

P17948

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