Recombinant human CD16a/FCGR3A protein | NKMAX ATGP4098 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human CD16a/FCGR3A protein

catalog :

ATGP4098

quantity :

10 ug

price :

120 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human CD16a/FCGR3A protein

Domain :

18-208aa

Catalog number (SKU) :

ATGP4098

NCBI :

NP_001121065.1

Description :

Fc gamma RIIIA/CD16a is also known as Low affinity immunoglobulin gamma Fc region receptor III-A isoform c. It is optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC). Also mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation. Involved in the generation of memory-like adaptive NK cells capable to produce high amounts of IFNG and to efficiently eliminate virus-infected cells via ADCC. Regulates NK cell survival and proliferation, in particular by preventing NK cell progenitor apoptosis. Fc gamma RIIIA is mediates the antitumor activities of therapeutic antibodies. Upon ligation on monocytes triggers TNFA-dependent ADCC of IgG-coated tumor cells. Recombinant human Fc gamma RIIIA/CD16a, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names :

Low affinity immunoglobulin gamma Fc region receptor III-A,low affinity immunoglobulin gamma Fc region receptor III-A isoform c, IgG Fc receptor III-A, CD16-II, CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcgammaRIIIA, FcR-10, IgG Fc receptor III-2, CD16a, FCGR3A, CD16A, FCG3, FCGR3, FCGRIII, IGFR3, IMD20, CD16

Concentration :

0.5mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Lee J., et al, (2015) Immunity. 42(3):431-442.;; DiLillo D.J., et al, (2014) Nat Med. 20(2):143-151.;; Warren H.S., et al, (1999) J Immunol. 162(2):735-742.;; Grier J.T., et al, (2012) J Clin Invest. 122(10):3769-3780.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured by its binding ability in a functional ELISA with Human IgG1 Fc (CAT# ATGP4094).

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Immunology

Molecular Weight :

22.6kDa (197aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human Fc gamma RIIIA/CD16a is coated at 10 ug/ml (100 ul/well) can bind Human IgG1 Fc (CAT# ATGP4094) in a functional ELISA assay.

Amino Acid Sequence :

MRTEDLPKAV VFLEPQWYRV LEKDSVTLKC QGAYSPEDNS TQWFHNESLI SSQASSYFID AATVDDSGEY RCQTNLSTLS DPVQLEVHIG WLLLQAPRWV FKEEDPIHLR CHSWKNTALH KVTYLQNGKG RKYFHHNSDF YIPKATLKDS GSYFCRGLFG SKNVSSETVN ITITQGLAVS TISSFFPPGY Q

Tag :

His-Tag

Expression System :

HEK293

Predicted nterm :

Met 18

Product Type Name :

Recombinant Protein

UniProt No. :

P08637

Internal Search Terms :

ATGP4098-10ug,ATGP4098-20ug,ATGP4098-50ug,ATGP4098-100ug,ATGP4098-250ug,ATGP4098-500ug,ATGP4098-1mg,ATGP4098-10,ATGP4098-20,ATGP4098-50,ATGP4098-100,ATGP4098-250,ATGP4098-500,ATGP4098-1

more info or order :

NKMAX product webpage