Recombinant human FGF-8b protein | NKMAX ATGP4097 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human FGF-8b protein

catalog :

ATGP4097

quantity :

10 ug

price :

130 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human FGF-8b protein

Domain :

23-215aa

Catalog number (SKU) :

ATGP4097

NCBI :

NP_006110.1

Description :

FGF-8b, also known as Fibroblast growth factor 8 isoform B, is a member of the fibroblast growth factor (FGF) family that plays a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. There are 4 known alternate spliced forms of FGF-8; FGF-8a, FGF-8b, FGF-8e and FGF-8f. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It encodes a key signaling factor, and its precise regulation is essential for embryo patterning. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity. FGF-8 signaling from the apical ectodermal ridge (AER), which borders the distal end of the limb bud, is necessary for forming normal limbs. FGF-8 is responsible for cell proliferation and survival, as well. The ability for FGF-8 to regulate cell proliferation has caused interest in its effects on tumors or squamous cell carcinoma. Recombinant human FGF-8b, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names :

FGF8, AIGF, Androgen-induced growth factor, FGF-8, HBGF-8, Heparin-binding growth factor 8, HH6, KAL6,fibroblast growth factor 8, fibroblast growth factor 8 isoform B

Concentration :

0.25 mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Tanaka, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8928-8932.;; Ornitz DM, et al. (1996). The Journal of Biological Chemistry. 271: 15292–15297.;; Crossley, P.H. et al. (1996) Cell, 84:127-136.;; Estienne A, et al.(2018) Reproduction. 155:53-62.;;

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells in the presence of 10 ug/ml of heparin. The ED50 range ≤ 5 ug/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Angiogenesis, Cardiovascular

Molecular Weight :

23.7kDa (204aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human FGF-8b stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cells in the presence of 10 ug/ml of heparin. The ED50 range ≤ to 5 ug/ml.

Amino Acid Sequence :

QVTVQ SSPNFTQHVR EQSLVTDQLS RRLIRTYQLY SRTSGKHVQV LANKRINAMA EDGDPFAKLI VETDTFGSRV RVRGAETGLY ICMNKKGKLI AKSNGKGKDC VFTEIVLENN YTALQNAKYE GWYMAFTRKG RPRKGSKTRQ HQREVHFMKR LPRGHHTTEQ SLRFEFLNYP PFTRSLRGSQ RTWAPEPR

Tag :

His-Tag

Expression System :

HEK293

Predicted nterm :

Asp

Product Type Name :

Recombinant Protein

UniProt No. :

P55075

Internal Search Terms :

ATGP4097-10ug,ATGP4097-20ug,ATGP4097-50ug,ATGP4097-100ug,ATGP4097-250ug,ATGP4097-500ug,ATGP4097-1mg,ATGP4097-10,ATGP4097-20,ATGP4097-50,ATGP4097-100,ATGP4097-250,ATGP4097-500,ATGP4097-1

more info or order :

NKMAX product webpage