Recombinant human CD14 protein | NKMAX ATGP4095 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human CD14 protein

catalog :

ATGP4095

quantity :

10 ug

price :

110 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human CD14 protein

Domain :

20-344aa

Catalog number (SKU) :

ATGP4095

NCBI :

NP_001167576.1

Description :

CD14, also known as monocyte differentiation antigen CD14, is a member of the eucine-rich repeat (LRR) proteins family. It exists in two forms, one anchored to the membrane by a GPI-tail (mCD14), the other a soluble form (sCD14). It is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide(LPS). It acts as a co-receptor (along with TLR4 and MD-2) and helps to detect bacteria in the body by binding LPS, a pathogen-associated molecular pattern (PAMP). Recombinant human CD14, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names :

monocyte differentiation antigen CD14, CD14 molecule, Myeloid cell-specific leucine-rich glycoprotein

Concentration :

1mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kelley S.L., et al (2013) J. Immunol. 190:1304-1311.;; Setoguchi M., et al (1989) Biochim. Biophys. Acta 1008:213-222.;;

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured by its ability to enhance IL-8 secretion using THP‑1 human acute monocytic leukemia cells in the presence of LPS. The ED50 range ≤ 100 ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Immunology

Molecular Weight :

35.8kDa (331aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human CD14 enhance the IL-8 secretion of the THP‑1 human acute monocytic leukemia cells in the presence of LPS. The ED50 range ≤ 100 ng/ml.

Amino Acid Sequence :

TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSM

Tag :

His-Tag

Expression System :

HEK293

Predicted nterm :

Thr 20

Product Type Name :

Recombinant Protein

UniProt No. :

P08571

Internal Search Terms :

ATGP4095-10ug,ATGP4095-20ug,ATGP4095-50ug,ATGP4095-100ug,ATGP4095-250ug,ATGP4095-500ug,ATGP4095-1mg,ATGP4095-10,ATGP4095-20,ATGP4095-50,ATGP4095-100,ATGP4095-250,ATGP4095-500,ATGP4095-1

more info or order :

NKMAX product webpage