protein

Recombinant human IgG1 Fc protein

ATGP4094

10 ug

72 USD

Recombinant human IgG1 Fc protein

100-330aa

ATGP4094

CAC20454.1

Immunoglobulins are membrane-bound or secreted glycoproteins produced by B lymphocytes. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity. IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors on phagocytic cells with high affinity. IgG comprise two identical light (L) and heavy (H) chains forming a ‘Y‐shaped’ structure. It also is digested by pepsin proteolysis into two distinct fragments (Fab, Fc). Fc fragment is a homodimeric glycoprotein with one monomer consisting of two constant immunoglobulin domains (CH2, CH3) from the C‐terminal half of one heavy chain. Through interaction with its ligands (FcγR, C1q and FcRn) it mediates various immune effector functions and increases the half‐life of the antibody molecule. Recombinant IgG Fc is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases. Recombinant human IgG1 Fc was expressed in HEK293 cell and purified by using conventional chromatography techniques.

immunoglobulin heavy chain constant region gamma 1, IGHG1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Michael McHeyzer-Williams., et al, (2012) Nat. Rev. Immunol. 12:24-34.;; Schroeder H.W. Jr., et al, (2010) J. Allergy Clin. Immunol. 125:S41-S52.;; E D Carosella., et al, (1988) Cell Immunol. 112:262-270.

> 90% by SDS-PAGE

1. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4102). The ED50 range ≤ 1.5 ug/ml.;; 2. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4116).;; 3. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4098). The ED50 range ≤ 4 ug/ml.

SDS-PAGE, Bioactivity

Human

Immunology

25.9kDa (231aa)

< 1 EU per 1ug of protein (determined by LAL method)

Human Fc gamma RIIIA/CD16a (CAT# ATGP4102) is coated at 10 ug/ml (100 ul/well) can bind Human IgG1 Fc. The ED50 range ≤ 1.5 ug/ml.Human IgG1 Fc is coated at 1ug/ml (100 ul/well) can bind Human Fc gamma RIIA/CD32a (H167) (CAT# ATGP4116) in a Functional ELISA assay.{2208220001306487.jpg}Human Fc gamma RIIIA/CD16a (CAT# ATGP4098) is coated at 10 ug/ml (100 ul/well) can bind Human IgG1 Fc. The ED50 range ≤ 4 ug/ml.{2208220001402774.jpg}

PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K

Non-Tagged

HEK293

Pro 100

Recombinant Protein

P01857

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