protein

Recombinant human PRCP protein

ATGP4088

10 ug

150 USD

Recombinant human PRCP protein

22-496aa

ATGP4088

NP_005031.1

PRCP, also known as prolylcarboxypeptidase, is a member of the peptidase S28 family of serine exopeptidases. This protein is proteolytically processed to generate the mature lysosomal prolylcarboxypeptidase. This enzyme cleaves C-terminal amino acids linked to proline in peptides such as angiotension 2, 3 and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. It has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension 2, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that PRCP may be related to essential hypertension. Recombinant human PRCP, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Prolylcarboxypeptidase, HUMPCP, PCP, lysosomal Pro-X carboxypeptidase isoform1, Angiotensinase C, Lysosomal carboxypeptidase C, Proline carboxypeptidase

0.25mg/ml (determined by Absorbance at 280nm)

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Diano S. (2011) Front Neuroendocrinol. 32:70-83.;; hagedorn M. (2013) Blood. 122:1337-1338.;; Duan L., et al, (2014) J Biol Chem. 289:21694-21705.;;

> 95% by SDS-PAGE

Specific activity is > 3,000 pmol/min/μg, and is defined as the amount of enzyme that convert 1.0 pmole of Z-Pro-Ala-OH per minute at pH 4.0 at 25C.

SDS-PAGE, Enzyme Activity

Human

Enzymes & Proteases, Ubiquitination

54.3kDa (481aa)

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 3,000 pmol/min/μg, and is defined as the amount of enzyme that convert 1.0 pmole of Z-Pro-Ala-OH per minute at pH 4.0 at 25C.

LRPALRALGS LHLPTNPTSL PAVAKNYSVL YFQQKVDHFG FNTVKTFNQR YLVADKYWKK NGGSILFYTG NEGDIIWFCN NTGFMWDVAE ELKAMLVFAE HRYYGESLPF GDNSFKDSRH LNFLTSEQAL ADFAELIKHL KRTIPGAENQ PVIAIGGSYG GMLAAWFRMK YPHMVVGALA ASAPIWQFED LVPCGVFMKI VTTDFRKSGP HCSESIHRSW DAINRLSNTG SGLQWLTGAL HLCSPLTSQD IQHLKDWISE TWVNLAMVDY PYASNFLQPL PAWPIKVVCQ YLKNPNVSDS LLLQNIFQAL NVYYNYSGQV KCLNISETAT SSLGTLGWSY QACTEVVMPF CTNGVDDMFE PHSWNLKELS DDCFQQWGVR PRPSWITTMY GGKNISSHTN IVFSNGELDP WSGGGVTKDI TDTLVAVTIS EGAHHLDLRT KNALDPMSVL LARSLEVRHM KNWIRDFYDS AGKQ

His-Tag

HEK293

Leu21

Recombinant Protein

P42785

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