protein

Recombinant Human Cathepsin D protein

ATGP4086

10 ug

150 USD

Recombinant Human Cathepsin D protein

21-412aa

ATGP4086

NP_001900.1

Cathepsin D, also known as lysosomal aspartyl protease, is a member of the peptidase C1 family, which is a normal and major component of lysosomes, and is found in almost all cells and tissues of mammals. The main physiological functions of cathepsin D consist of metabolic degradation of intracellular proteins, activation and degradation of polypeptide hormones and growth factors, activation of enzymatic precursors, processing of enzyme activators and inhibitors, brain antigen processing and regulation of programmed cell death. In addition, it secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogenous inhibitor of angiogenesis, suggesting its contribution to the prevention of tumor growth and angiogenesis-dependent growth of metastases. Recombinant human Cathepsin D, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

CTSD, CPSD, Cathepsin D, Lysosomal aspartyl protease, CLN10, Ceroid-lipofuscinosis, Neuronal 10

1 mg/ml (determined by Absorbance at 280nm)

Liquid in. 50mM MES buffer (pH 5.5) containing 100mM NaCl, 20% glycerol.

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Fusek M, et al. (2005). 149: 43-50.;; Minarowska A, et al. (2007)l. 45: 159-163.;; Tsukuba, et al. (2000) Mol. Cells 10:601-611.;;

> 95% by SDS-PAGE

Specific activity is > 20 pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-PLGL-Dpa-AR-NH2 per minute at pH 3.5 at 25C.

SDS-PAGE, Enzyme Activity

Human

Adhesion & Movement, Neuroscience

43.4 kDa (398aa)

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 20 pmol/min/ug, and is defined as the amount of enzyme that cleaves 1pmol of Mca-PLGL-Dpa-AR-NH2 per minute at pH 3.5 at 25C.

LVRIPLHKFT SIRRTMSEVG GSVEDLIAKG PVSKYSQAVP AVTEGPIPEV LKNYMDAQYY GEIGIGTPPQ CFTVVFDTGS SNLWVPSIHC KLLDIACWIH HKYNSDKSST YVKNGTSFDI HYGSGSLSGY LSQDTVSVPC QSASSASALG GVKVERQVFG EATKQPGITF IAAKFDGILG MAYPRISVNN VLPVFDNLMQ QKLVDQNIFS FYLSRDPDAQ PGGELMLGGT DSKYYKGSLS YLNVTRKAYW QVHLDQVEVA SGLTLCKEGC EAIVDTGTSL MVGPVDEVRE LQKAIGAVPL IQGEYMIPCE KVSTLPAITL KLGGKGYKLS PEDYTLKVSQ AGKTLCLSGF MGMDIPPPSG PLWILGDVFI GRYYTVFDRD NNRVGFAEAA RL

His-Tag

HEK293

Leu21

Recombinant Protein

P07339

ATGP4086-10ug,ATGP4086-20ug,ATGP4086-50ug,ATGP4086-100ug,ATGP4086-250ug,ATGP4086-500ug,ATGP4086-1mg,ATGP4086-10,ATGP4086-20,ATGP4086-50,ATGP4086-100,ATGP4086-250,ATGP4086-500,ATGP4086-1