Recombinant rat TrkA protein | NKMAX ATGP4085 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant rat TrkA protein

catalog :

ATGP4085

quantity :

10 ug

price :

130 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant rat TrkA protein

Domain :

35-418aa

Catalog number (SKU) :

ATGP4085

NCBI :

NP_067600.1

Description :

TrkA, also known as Tyrosine kinase receptor A, is a member of the neurotrophic tyrosine kinase receptor(NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. It leads to cell differentiation and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as the establishment of thermal regulation via sweating. In one study conducted on two rat models, an inhibition of TrkA with AR786 led to a reduction in joint swelling, joint damage, and pain caused by inflammatory arthritis. Recombinant rat TrkA, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names :

High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, NTRK-1, NTRK1, Slow nerve growth factor receptor, p140-TrkA, Trk-A, Trk, Trka, TRK1-transforming tyrosine kinase protein, TRKAOncogene TRK, TRKTRK1, tyrosine kinase receptor A

Concentration :

1 mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Benito-Gutiérrez E, et al. (2006). Mol Cell Neurosci. 31:179-192.;; Lambiase A, et al. (2005). Natl Acad Sci. 102:16795-16800.

Purity :

> 90% by SDS - PAGE

Bioactivity Comment Only :

Measured by ability to inhibit NGF-induced proliferation assay using TF‑1 human erythroleukemic cells in the presence of 0.5ng/ml of rat NGF. The ED50 range ≤ 5 ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Rat

Gene Category :

Neuroscience

Molecular Weight :

69kDa (623aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Rat Ntrk1 in inhibit the cell growth using TF-1 human erythroleukemic cells in the presence of 0.5ng/ml of rat NGF. The ED50 range ≤ 5ng/ml.

Amino Acid Sequence :

SCRETCCPVG PSGLRCTRAG TLNTLRGLRG AGNLTELYVE NQRDLQRLEF EDLQGLGELR SLTIVKSGLR FVAPDAFHFT PRLSHLNLSS NALESLSWKT VQGLSLQDLT LSGNPLHCSC ALLWLQRWEQ EDLCGVYTQK LQGSGSGDQF LPLGHNNSCG VPSVKIQMPN DSVEVGDDVF LQCQVEGQAL QQADWILTEL EGTATMKKSG DLPSLGLTLV NVTSDLNKKN VTCWAENDVG RAEVSVQVSV SFPASVHLGK AVEQHHWCIP FSVDGQPAPS LRWFFNGSVL NETSFIFTQF LESALTNETM RHGCLRLNQP THVNNGNYTL LAANPYGQAA ASIMAAFMDN PFEFNPEDPI PVSFSPVDTN STSRDPVEKK DETP

Tag :

hIgG-His-Tag

Expression System :

HEK293

Predicted nterm :

Ser 35

Product Type Name :

Recombinant Protein

UniProt No. :

P35739

Internal Search Terms :

ATGP4085-10ug,ATGP4085-20ug,ATGP4085-50ug,ATGP4085-100ug,ATGP4085-250ug,ATGP4085-500ug,ATGP4085-1mg,ATGP4085-10,ATGP4085-20,ATGP4085-50,ATGP4085-100,ATGP4085-250,ATGP4085-500,ATGP4085-1

more info or order :

NKMAX product webpage