protein

Recombinant human ALT2/GPT2 protein

ATGP4079

10 ug

150 USD

Recombinant human ALT2/GPT2 protein

1-523aa

ATGP4079

NP_597700

Alanine aminotransferase 2, also known as GPT2, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Subsequently, they play a key role in the intermediary metabolism of glucose and amino acids. GPT2 expression is high in muscle, fat and kidney. Recombinant human GPT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, GPT 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2, AAT2, ALT2

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 30% glycerol, 2mM DTT, 0.2M NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Sohocki M.M. et al. (1997) Genomics 40: 247-252.;; Matthews C.C. et al. (2003) Hepatology. 39: 1297-1302.

> 90% by SDS-PAGE

Specific activity is > 100unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C

SDS-PAGE, Enzyme Activity

Human

Metabolism

60.3 kDa (546aa)

Specific activity is > 100units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C

MQRAAAL VRRGCGPRTP SSWGRSQSSA AAEASAVLKV RPERSRRERI LTLESMNPQV KAVEYAVRGP IVLKAGEIEL ELQRGIKKPF TEVIRANIGD AQAMGQQPIT FLRQVMALCT YPNLLDSPSF PEDAKKRARR ILQACGGNSL GSYSASQGVN CIREDVAAYI TRRDGGVPAD PDNIYLTTGA SDGISTILKI LVSGGGKSRT GVMIPIPQYP LYSAVISELD AIQVNYYLDE ENCWALNVNE LRRAVQEAKD HCDPKVLCII NPGNPTGQVQ SRKCIEDVIH FAWEEKLFLL ADEVYQDNVY SPDCRFHSFK KVLYEMGPEY SSNVELASFH STSKGYMGEC GYRGGYMEVI NLHPEIKGQL VKLLSVRLCP PVSGQAAMDI VVNPPVAGEE SFEQFSREKE SVLGNLAKKA KLTEDLFNQV PGIHCNPLQG AMYAFPRIFI PAKAVEAAQA HQMAPDMFYC MKLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPVEKLKTV LQKVKDFHIN FLEKYA

His-Tag

E.coli

Recombinant Protein

Q8TD30

AAT 2, AAT2, Alanine aminotransferase 2, ALT 2, ALT2, Glutamate pyruvate transaminase 2, Glutamic alanine transaminase 2, Glutamic-alanine transaminase 2, Glutamic--alanine transaminase 2, Glutamic-pyruvic transaminase 2, Glutamic--pyruvic transaminase 2, GPT 2, GPT2, Glutamic pyruvic transaminase 2, ATGP4079-10ug, ATGP4079-20ug, ATGP4079-50ug, ATGP4079-100ug, ATGP4079-250ug, ATGP4079-500ug, ATGP4079-1mg, ATGP4079-010, ATGP4079-020, ATGP4079-050, ATGP4079-100, ATGP4079-250, ATGP4079-500, ATGP4079-01M