Recombinant human CD47 protein | NKMAX ATGP4070 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human CD47 protein

catalog :

ATGP4070

quantity :

10 ug

price :

110 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human CD47 protein

Domain :

19-141aa

Catalog number (SKU) :

ATGP4070

NCBI :

NP_001768

Description :

CD47, also known as leukocyte surface antigen CD47 isoform 1, is a member of immunoglobulin superfamily. It has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Also, it plays an important role in memory formation and synaptic plasticity in the hippocampus. This protein is a receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 mediates cell-cell adhesion, enhances super antigen-dependent T-cell-mediated proliferation and co-stimulates T-cell activation. It may play a role in membrane transport and/or integrin dependent signal transduction and prevent premature elimination of red blood cells. Recombinant human CD47, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Leukocyte surface antigen CD47 isoform 1, CD47, IAP, MER6, OA3

Concentration :

0.5mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Iwamoto C., et al. (2014) Exp. Hematol. 42:163-171.;; Wang Y., et al. (2013) Mol. Ther. 21:1919-1929.;; Jiang H., et al. (2013) Leuk. Res. 37:907-910.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured by its binding ability in a functional ELISA with Human SIRP alpha/CD172a (CAT# ATGP4069). The ED50 range ≤ 100 ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Immunology, Adhesion & Movement

Molecular Weight :

40.9 kDa (362aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human SIRP alpha/CD172a (CAT# ATGP4069) is coated at 2 ug/ml (100 ul/well) can bind Human CD47. The ED50 range ≤ 100 ng/ml.

Amino Acid Sequence :

QLLFNKTKSV EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP TDFSSAKIEV SQLLKGDASL KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS PNE

Tag :

hIgG-His-Tag

Expression System :

Baculovirus

Predicted nterm :

Gln19

Product Type Name :

Recombinant Protein

UniProt No. :

Q08722

Internal Search Terms :

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more info or order :

NKMAX product webpage