Recombinant human SIRP alpha/CD172a protein | NKMAX ATGP4069 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human SIRP alpha/CD172a protein

catalog :

ATGP4069

quantity :

10 ug

price :

130 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human SIRP alpha/CD172a protein

Domain :

27-373aa

Catalog number (SKU) :

ATGP4069

NCBI :

NP_542970.1

Description :

SIRP alpha is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRP alpha interacts with a broadly expressed transmembrane protein CD47. This interaction negatively controls effector function of innate immune cells such as host cell phagocytosis. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. It was found to participate in signal transduction mediated by various growth factor receptors. Recombinant human SIRP alpha, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names :

SIRPA, Tyrosine-protein phosphatase non-receptor type substrate 1 isoform 1, SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Bit, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha-1, Sirp-alpha-1, Signal-regulatory protein alpha-2, Sirp-alpha-2, Signal-regulatory protein alpha-3, Sirp-alpha-3, p84, CD172a, BIT, MFR, MYD1, PTPNS1, SHPS1,SIRP

Concentration :

1mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Timms J.F., Swanson K.D., et al. (1999) Curr. Biol. 9:927-930.;; Barclay, A.N. & M.H. Brown (2006) Nat. Rev. Immunol. 6:457.

Purity :

> 95% by SDS - PAGE

Bioactivity Comment Only :

Measured by its binding ability in a functional ELISA with Human CD47 (CAT# ATGP4070).

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Adhesion & Movement, Neuroscience

Molecular Weight :

39kDa (356aa)

Bioactivity Comment and Pics :

Human SIRP alpha/CD172a is coated at 2 ug/ml (100 ul/well) can bind Human CD47 (CAT# ATGP4070) in a Functional ELISA assay.

Amino Acid Sequence :

GVAGEEE LQVIQPDKSV LVAAGETATL RCTATSLIPV GPIQWFRGAG PGRELIYNQK EGHFPRVTTV SDLTKRNNMD FSIRIGNITP ADAGTYYCVK FRKGSPDDVE FKSGAGTELS VRAKPSAPVV SGPAARATPQ HTVSFTCESH GFSPRDITLK WFKNGNELSD FQTNVDPVGE SVSYSIHSTA KVVLTREDVH SQVICEVAHV TLQGDPLRGT ANLSETIRVP PTLEVTQQPV RAENQVNVTC QVRKFYPQRL QLTWLENGNV SRTETASTVT ENKDGTYNWM SWLLVNVSAH RDDVKLTCQV EHDGQPAVSK SHDLKVSAHP KEQGSNTAAE NTGSNERNIY

Tag :

His-Tag

Expression System :

HEK293

Predicted nterm :

Asp

Product Type Name :

Recombinant Protein

UniProt No. :

P78324

Internal Search Terms :

ATGP4069-10ug,ATGP4069-20ug,ATGP4069-50ug,ATGP4069-100ug,ATGP4069-250ug,ATGP4069-500ug,ATGP4069-1mg,ATGP4069-10,ATGP4069-20,ATGP4069-50,ATGP4069-100,ATGP4069-250,ATGP4069-500,ATGP4069-1

more info or order :

NKMAX product webpage