protein

Recombinant human NKp80/KLRF1 protein

ATGP4063

10 ug

130 USD

Recombinant human NKp80/KLRF1 protein

60-231aa

ATGP4063

NP_057607.1

NKp80/KLRF1, also known as killer cell lectin-like receptor subfamily F member 1, is a member of the C-type lectin family. Multiple splicing variants produce four isoforms for NKp80/KLRF1 and isoform 1 is the standard protein. It is expressed on NK cells and a subset of T lymphocytes whereas it is not expressed on B lymphocytes, monocytes and granulocytes. It stimulates NK cell cytotoxicity and induces calcium influx after triggering by appropriate antibodies. It works through interaction with its ligand, AICL (activation-induced C-type lectin), which is selectively expressed on myeloid cells. NKp80-AICL interaction regulates the immune responses at sites of inflammation by stimulating the release of proinflammatory cytokines. Recombinant human NKp80/KLRF1, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Killer cell lectin-like receptor F1, Killer cell lectin-like receptor subfamily F member 1,Killer cell lectin-like receptor subfamily F member 1 isoform 1, Lectin-like receptor F1, Activating coreceptor NKp80, NKp80, KLRF1, C-type lectin domain family 5 member C, CLEC5C, ML, CLEC5CMGC119908, MGC119907, MGC119909

0.25mg/ml (determined by Absorbance at 280nm)

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Welte S. et al. (2006) Nat. Immunol. 7:1334-1342.;; Roda-Navarro P. et al. (2000) Eur. J. Immunol. 30:568–576.

> 85% by SDS - PAGE

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to human KLRF1 coated plates 2 ug/ml. This effect is more to 60%.

SDS-PAGE, Bioactivity

Human

Immunology

47.1kDa (414aa)

< 1 EU per 1ug of protein (determined by LAL method)

Human KLRF1 stimulates cell adhesion of the U937 human histiocytic lymphoma cells. This effect is more to 60%.

LLVSQGV LLKCQKGSCS NATQYEDTGD LKVNNGTRRN ISNKDLCASR SADQTVLCQS EWLKYQGKCY WFSNEMKSWS DSYVYCLERK SHLLIIHDQL EMAFIQKNLR QLNYVWIGLN FTSLKMTWTW VDGSPIDSKI FFIKGPAKEN SCAAIKESKI FSETCSSVFK WICQY

hIgG-His-Tag

HEK293

Asp

Recombinant Protein

Q9NZS2

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