Recombinant human Acetylcholinesterase/ACHE protein | NKMAX ATGP4058 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Acetylcholinesterase/ACHE protein

catalog :

ATGP4058

quantity :

10 ug

price :

160 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Acetylcholinesterase/ACHE protein

Domain :

32-614aa

Catalog number (SKU) :

ATGP4058

NCBI :

NP_000656.1

Description :

ACHE, also known as Acetylcholinesterase, is a member of the type-B carboxylesterase/lipase family. It is an enzyme that catalyzes the breakdown of acetylcholine and of some other choline esters that function as neurotransmitters. During neurotransmission, ACH is released from the presynaptic neuron into the synaptic cleft and binds to ACH receptors on the post-synaptic membrane, relaying the signal from the nerve. ACHE, also located on the post-synaptic membrane, terminates the signal transmission by hydrolyzing ACH. This protein is thought to be involved in the pathology of Alzheimers disease (AD) by accelerating the assembly of A beta peptides into fibrillar species through forming complexes with A beta via the peripheral anionic site on ACHE. ACHE inhibitors have been used to delay symptoms of AD patients by virtue of their ability to enhance ACH availability, as well as reduce amyloidogenesis and subsequent neurotoxicity. Recombinant human ACHE, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names :

AChE, ACEE, ACES_HUMAN, Acetylcholinesterase, ACHE, ARACHE, N-ACHE, VT, Acetylcholinesterase isoform E4-E6

Concentration :

0.25mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Grisaru, D. et al, (1999) Eur. J. Biochem. 264:672-686.;; Harry M. Greenblatt et al, (2003) J. Mol. Neurosci. 20:369–383.

Purity :

> 95% by SDS – PAGE

Bioactivity Comment Only :

Specific activity is > 6,000 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1.0 nmol acetylthiocholine per minute at pH7.5 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Neuroscience

Molecular Weight :

65.6kDa (592aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 6,000 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1.0 nmol acetylthiocholine per minute at pH7.5 at 25C.

Amino Acid Sequence :

EGREDAE LLVTVRGGRL RGIRLKTPGG PVSAFLGIPF AEPPMGPRRF LPPEPKQPWS GVVDATTFQS VCYQYVDTLY PGFEGTEMWN PNRELSEDCL YLNVWTPYPR PTSPTPVLVW IYGGGFYSGA SSLDVYDGRF LVQAERTVLV SMNYRVGAFG FLALPGSREA PGNVGLLDQR LALQWVQENV AAFGGDPTSV TLFGESAGAA SVGMHLLSPP SRGLFHRAVL QSGAPNGPWA TVGMGEARRR ATQLAHLVGC PPGGTGGNDT ELVACLRTRP AQVLVNHEWH VLPQESVFRF SFVPVVDGDF LSDTPEALIN AGDFHGLQVL VGVVKDEGSY FLVYGAPGFS KDNESLISRA EFLAGVRVGV PQVSDLAAEA VVLHYTDWLH PEDPARLREA LSDVVGDHNV VCPVAQLAGR LAAQGARVYA YVFEHRASTL SWPLWMGVPH GYEIEFIFGI PLDPSRNYTA EEKIFAQRLM RYWANFARTG DPNEPRDPKA PQWPPYTAGA QQYVSLDLRP LEVRRGLRAQ ACAFWNRFLP KLLSATDTLD EAERQWKAEF HRWSSYMVHW KNQFDHYSKQ DRCSDL

Tag :

His-Tag

Expression System :

HEK293

Predicted nterm :

Asp

Product Type Name :

Recombinant Protein

UniProt No. :

P22303

Internal Search Terms :

ATGP4058-10ug,ATGP4058-20ug,ATGP4058-50ug,ATGP4058-100ug,ATGP4058-250ug,ATGP4058-500ug,ATGP4058-1mg,ATGP4058-10,ATGP4058-20,ATGP4058-50,ATGP4058-100,ATGP4058-250,ATGP4058-500,ATGP4058-1

more info or order :

NKMAX product webpage