Recombinant human ENPP-2/Autotaxin protein | NKMAX ATGP4057 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human ENPP-2/Autotaxin protein

catalog :

ATGP4057

quantity :

10 ug

price :

160 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human ENPP-2/Autotaxin protein

Domain :

49-863aa

Catalog number (SKU) :

ATGP4057

NCBI :

NP_001035181.1

Description :

ENPP-2, also known as Autotaxin, belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. ENPP-2 is able to cleave the phosphodiester bond between the α and the β position of triphosphate nucleotides, acting as an ectonucleotide phosphodiesterase producing pyrophosphate , as most members of the ENPP family. It is unlike ENPP-1 and ENPP-3, has weak activity against nucleotides, but exhibits a lysophospholipase D activity which allows the formation of lysophosphatidic acid (LPA) and choline from lysophosphatidylcholine. Also, ENPP-2 and LPA are involved in numerous inflammatory-driven diseases such as asthma and arthritis. Recombinant human ENPP-2/Autotaxin, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Alternative Names :

Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 isoform 2, ectonucleotide pyrophosphatase/phosphodiesterase 2, ENPP2, E-NPP 2, AUTOTAXIN, Extracellular lysophospholipase D, LysoPLD, ENPP2, ATX, PDNP2, ATX-X, NPP2, PD-IALPHA

Concentration :

0.25mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles

General References :

Benesch MG., et al, (2014) FEBS Letters. 588:2712-2727.;; Kawagoe H., et al, (1995) Genomics. 30:380-384.;; Benesch MG., et al, (2015) Journal of Lipid Research. 56:1134-1144.

Purity :

> 90% by SDS - PAGE

Bioactivity Comment Only :

Specific activity is > 15,000 units/mg, and defined as the amount of enzyme that hydrolyze 1nmole of bis(p-Nitrophenyl) phosphate per minute at pH8.7 at 37℃.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Cardiovascular

Molecular Weight :

94.9kDa (825aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 15,000 units/mg, and defined as the amount of enzyme that hydrolyze 1nmole of bis(p-Nitrophenyl) phosphate per minute at pH8.7 at 37℃.

Amino Acid Sequence :

MDSPWTN ISGSCKGRCF ELQEAGPPDC RCDNLCKSYT SCCHDFDELC LKTARGWECT KDRCGEVRNE ENACHCSEDC LARGDCCTNY QVVCKGESHW VDDDCEEIKA AECPAGFVRP PLIIFSVDGF RASYMKKGSK VMPNIEKLRS CGTHSPYMRP VYPTKTFPNL YTLATGLYPE SHGIVGNSMY DPVFDATFHL RGREKFNHRW WGGQPLWITA TKQGVKAGTF FWSVVIPHER RILTILQWLT LPDHERPSVY AFYSEQPDFS GHKYGPFGPE MTNPLREIDK IVGQLMDGLK QLKLHRCVNV IFVGDHGMED VTCDRTEFLS NYLTNVDDIT LVPGTLGRIR SKFSNNAKYD PKAIIANLTC KKPDQHFKPY LKQHLPKRLH YANNRRIEDI HLLVERRWHV ARKPLDVYKK PSGKCFFQGD HGFDNKVNSM QTVFVGYGST FKYKTKVPPF ENIELYNVMC DLLGLKPAPN NGTHGSLNHL LRTNTFRPTM PEEVTRPNYP GIMYLQSDFD LGCTCDDKVE PKNKLDELNK RLHTKGSTEE RHLLYGRPAV LYRTRYDILY HTDFESGYSE IFLMPLWTSY TVSKQAEVSS VPDHLTSCVR PDVRVSPSFS QNCLAYKNDK QMSYGFLFPP YLSSSPEAKY DAFLVTNMVP MYPAFKRVWN YFQRVLVKKY ASERNGVNVI SGPIFDYDYD GLHDTEDKIK QYVEGSSIPV PTHYYSIITS CLDFTQPADK CDGPLSVSSF ILPHRPDNEE SCNSSEDESK WVEELMKMHT ARVRDIEHLT SLDFFRKTSR SYPEILTLKT YLHTYESEI

Tag :

His-Tag

Expression System :

HEK293

Predicted nterm :

Asp

Product Type Name :

Recombinant Protein

UniProt No. :

Q13822

Internal Search Terms :

ATGP4057-10ug,ATGP4057-20ug,ATGP4057-50ug,ATGP4057-100ug,ATGP4057-250ug,ATGP4057-500ug,ATGP4057-1mg,ATGP4057-10,ATGP4057-20,ATGP4057-50,ATGP4057-100,ATGP4057-250,ATGP4057-500,ATGP4057-1

more info or order :

NKMAX product webpage