protein

Recombinant mouse ALCAM/CD166 protein

ATGP4055

10 ug

117 USD

Recombinant mouse ALCAM/CD166 protein

28-527aa

ATGP4055

NP_033785

ALCAM (Activated leukocyte cell adhesion molecule), also known as CD166 antigen, is a member of the Ig CAM family within the immunoglobulin superfamily. It interacts with CD6 that promotes T-cell activation and proliferation and plays a role in the binding of T- and B-cells to activated leukocytes. While expressed in a wide variety of tissues, ALCAM is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. Recently, CD166 is regarded as a potential novel breast cancer indicator and therapeutic target. Recombinant mouse ALCAM, fused to hIgG-His-tag at C-terminus, was expressed in Insect cell and purified by using conventional chromatography techniques.

Activated leukocyte cell adhesion molecule, ALCAM, Alcam, CD166 antigen, CD166 antigen isoform1, CD166, BEN, Protein DM-GRASP, AI853494 Protein, MuSC, SC1

0.25mg/ml (determined by Bradford assay)

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 50% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213-2220.;;Castro, M.A.A. et al. (2007) J. Immunol. 178:4351-4361.;;Degen, W.G. et al. (1998) Am. J. Pathol. 152:805-813.;;King, J.A. et al. (2010) Mol. Cancer 9:266.;;

> 90% by SDS - PAGE

Measured by its ability to block the adhesion of Jurkat human acute T cell leukemia cells to immobilized Recombinant Human CD6 (CAT# ATGP4005). When cells are added to 1 ug/ml of mouse ALCAM binded to CD6, This effect is more to 50%.

SDS-PAGE, Bioactivity

Mouse

Immunology

83.1kDa (739aa)

< 1 EU per 1ug of protein (determined by LAL method)

Mouse ALCAM stimulates inhibit cell adhesion of the Jurkat human acute T cell leukemia cells by CD6. This effect is more to 50%.

WYTVNSAYGD TIVMPCRLDV PQNLMFGKWK YEKPDGSPVF IAFRSSTKKS VQYDDVPEYK DRLSLSENYT LSIANAKISD EKRFVCMLVT EDNVFEAPTL VKVFKQPSKP EIVNKAPFLE TDQLKKLGDC ISRDSYPDGN ITWYRNGKVL QPVEGEVAIL FKKEIDPGTQ LYTVTSSLEY KTTRSDIQMP FTCSVTYYGP SGQKTIYSEQ EIFDIYYPTE QVTIQVLPPK NAIKEGDNIT LQCLGNGNPP PEEFMFYLPG QPEGIRSSNT YTLTDVRRNA TGDYKCSLID KRNMAASTTI TVHYLDLSLN PSGEVTKQIG DTLPVSCTIS ASRNATVVWM KDNIRLRSSP SFSSLHYQDA GNYVCETALQ EVEGLKKRES LTLIVEGKPQ IKMTKKTDPS GLSKTIICHV EGFPKPAIHW TITGSGSVIN QTEESPYING RYYSKIIISP EENVTLTCTA ENQLERTVNS LNVSAISIPE HDEADDISDE NREKVNDQAK

hIgG-His-Tag

Baculovirus

Trp28

Recombinant Protein

Q61490

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