Recombinant E.coli rnpA protein | NKMAX ATGP4054 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant E.coli rnpA protein

catalog :

ATGP4054

quantity :

10 ug

price :

130 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant E.coli rnpA protein

Domain :

1-119aa

Catalog number (SKU) :

ATGP4054

NCBI :

NP_418159.1

Description :

rnpA, also known as Rnase P protein, is an essential enzyme consisting of the C5 protein (encoded by rnpA) and the catalytic M1 RNA (encoded by rnpB) subunits. rnpA is ribonucleoprotein that catalyzes the removal of the 50-leader elements of precursor tRNAs and generates the mature 50-end of tRNAs. This step is essential for the formation of functional tRNA molecules in bacteria, archaea and eukarya. More importantly, it has recently been demonstrated that RNase P is required for the endonucleolytic separation of certain polycistronic tRNA transcripts such as valV valW, leuQ leuP leuV and secG leuU. Thus, it was hypothesized that the essential function of RNase P might be related to the complete absence of a particular tRNAthat was dependent on the enzyme for initial separation from polycistronic transcripts. Recombinant E.coli rnpA, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

ECK3696, Rnase P protein, RnaseP protein, b3704, JW3681, Ribonuclease P protein component, EC 3.1.26.5, Protein C5

Concentration :

0.25mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid. Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ankit Agrawal., et al. (2014) Nucleic Acids Res. 42(17):11166-79.;; Hui-Woog Choe., et al. (2002) Acta Crystallogr D Biol Crystallogr. 59(Pt 2):350-2.

Purity :

> 90% by SDS - PAGE

Applications :

SDS-PAGE

Antigen Species :

E.coli

Gene Category :

Epigenetics and Nuclear Signaling

Molecular Weight :

13.7kDa

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Amino Acid Sequence :

MVKLAFPREL RLLTPSQFTF VFQQPQRAGT PQITILGRLN SLGHPRIGLT VAKKNVRRAH ERNRIKRLTR ESFRLRQHEL PAMDFVVVAK KGVADLDNRA LSEALEKLWR RHCRLARGS

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P0A7Y8

Internal Search Terms :

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more info or order :

NKMAX product webpage