protein

Recombinant human Ribonuclease A/RNASE 1 protein

ATGP4051

10 ug

140 USD

Recombinant human Ribonuclease A/RNASE 1 protein

29-156aa

ATGP4051

NP_937878.1

RNASE1, also known as ribonuclease A, is a member of pancreatic ribonuclease enzyme family. It is a relatively small protein and is a basic protein (pI = 9.63). Also, It has four disulfide bonds in its native state. It cleaves specifically after pyrimidine nucleotides. Cleavage takes place in two steps: first, the 3’,5’-phosphodiester bond is cleaved to generate a 2’,3’-cyclic phosphodiester intermediate; second, the cyclic phosphodiester is hydrolyzed to a 3’-monophosphate. (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG) The highest activity is exhibited with single stranded RNA. It can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. Recombinant human RNASE1, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

RNASE1, ribonuclease A family member 1, pancreatic, RAC1, RIB1, RNS1, ribonuclease pancreatic, HP-RNase, RIB-1, RNase UpI-1, RNase 1, Ribonuclease A, RNase A

1mg/ml (determined by Absorbance at 280nm)

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Raines RT (1998) Chem. Rev. 98:1045–1066.;; JC Kosgey et al, (2020) Int. J. Biol. 160:1042-1049.

90% by SDS – PAGE

Specific activity is > 5 X 10^4 unit/mg, and is defined as the amount of enzyme that cleaves 1.0 pmole of RNase probe per minute at 25C.

SDS-PAGE, Enzyme Activity

Human

Enzymes & Proteases, Epigenomics (Transcription & Translation)

N- terminal Sequence Analysis: Lys-Glu-Ser-Arg-Ala

15.3kDa(134aa)

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 3 X 10^6 unit/mg, and is defined as the amount of enzyme that cleaves 1.0 pmole of RNase probe per minute at 25C.

KESRAKKFQR QHMDSDSSPS SSSTYCNQMM RRRNMTQGRC KPVNTFVHEP LVDVQNVCFQ EKVTCKNGQG NCYKSNSSMH ITDCRLTNGS RYPNCAYRTS PKERHIIVAC EGSPYVPVHF DASVEDST

His-Tag

HEK293

Lys29

Recombinant Protein

P07998

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