protein

Recombinant Mouse IL-21 Protein

ATGP4049

10 ug

143 USD

Recombinant Mouse IL-21 Protein

18-146aa

ATGP4049

NP_068554.1

IL-21, also known as Interleukin-21, is produced from activated helper T cells such as Th17 cells and is a member of the common-γ chain family of cytokines that includes IL-2, IL-4, IL-7, IL-9, and IL-15. Previous studies have shown that IL-21 plays an important role in the regulation of both innate and adaptive immune responses. IL-21 is critical for NK cell activation and expansion. IL-21 also promotes terminal B cell differentiation into plasma cells and antibody production. Furthermore, IL-21 enhances antigen-specific CD8 T cell expansion and the differentiation of follicular helper T cells in vivo. IL-21 also promotes the differentiation of the inflammatory Th17 lineage and contributes to autoimmunity. IL-21 isoform is an alternative splice variant form of IL-21 that we previously identified and reported to have a similar function as original IL-21. Follicular helper T cells also produce IL-21 to activate and differentiate B cells. Recombinant mouse IL-21, was expressed in E. coli and purified by using conventional chromatography techniques.

Interleukin-21, IL-21, CVID11, IL21, IL-21, IL-21Za11interleukin-21, Interleukin-21 isoform, Za11, UscroPMagleukin-21, IL-21, 2

1mg/ml (determined by Bradford assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Yuqing Yuan., et al. (2017) JCI Insight. 6;2(7):e90652.;; Akemi Araki., et al. (2019) J Immunol. 202:3326-3333.

> 90% by SDS - PAGE

The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤ 100 ng/ml.

SDS-PAGE, Bioactivity

Mouse

Hormones & Cytokines

15 kDa (130aa), confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Mouse IL-21 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range ≤ 100 ng/ml.

MHKSSPQGPD RLLIRLRHLI DIVEQLKIYE NDLDPELLSA PQDVKGHCEH AAFACFQKAK LKPSNPGNNK TFIIDLVAQL RRRLPARRGG KKQKHIAKCP SCDSYEKRTP KEFLERLKWL LQKMIHQHLS

Non-Tagged

E.coli

Recombinant Protein

Q9ES17

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