Recombinant mouse CTLA4 protein | NKMAX ATGP4046 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse CTLA4 protein

catalog :

ATGP4046

quantity :

10 ug

price :

56 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse CTLA4 protein

Domain :

38-161aa

Catalog number (SKU) :

ATGP4046

NCBI :

NP_033973.2

Description :

Ctla-4, also known as cytotoxic T-lymphocyte protein 4 isoform 1, is a type 1 membrane protein and a memberof the immunoglobulin-superfamily. Besides, this protein contains an extra cellular domain, a transmembranedomain and a cytoplasmic tail. It is generally expressed with highest levels in lymphoid tissues. Also, it is similarto T-cell costimulatory protein CD28 since both of the molecules bind to CD80 and CD86 on antigen-presenting cells. CTLA4 and CD28 effectively regulate to T cell responses as opposing signals transmitted through tworelated cell-surface receptors. Recombinant mouse Ctla-4, fused to hIgG-His-tag at C-terminus, was expressed ininsect cell and purified by using conventional chromatography techniques.

Alternative Names :

Ly-56, Ly-5, Cytotoxic T-lymphocyte-associated antigen 4, Cytotoxic T-lymphocyte protein 4 isoform 1, Ctla-4, CTLA4, CTLA, Cd152

Concentration :

0.5mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Krummey SM., et al. (2014) J. Immunol. 192:2495-2504.;; Jain N., et al. (2013) Nat. Med. 19:1632-1637.;; Yu CR., et al. (2013) J. Immunol. 191:5036-5043.

Purity :

> 90% by SDS - PAGE

Bioactivity Comment Only :

The activity is determined by the IL-2 ELISA in a using stimulated Jurkat human acute T cell leukemia cells with Human B7-1/CD80. The ED50 range ≤150ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Mouse

Gene Category :

Immunology

Molecular Weight :

40.6kDa (363aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Mouse Ctla4 inhibits IL-2 secretion of the stimulated Jurkat human acute T cell leukemia cells with Human B7-1/CD80. The ED50 range ≤150 ng/ml.

Amino Acid Sequence :

IQVTQPSVVL ASSHGVASFP CEYSPSHNTD EVRVTVLRQT NDQMTEVCAT TFTEKNTVGF LDYPFCSGTF NESRVNLTIQ GLRAVDTGLY LCKVELMYPP PYFVGMGNGT QIYVIDPEPC PDSD

Tag :

hIgG-His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ile 38

Product Type Name :

Recombinant Protein

UniProt No. :

P09793

Internal Search Terms :

ATGP4046-10ug,ATGP4046-20ug,ATGP4046-50ug,ATGP4046-100ug,ATGP4046-250ug,ATGP4046-500ug,ATGP4046-1mg, ATGP4046-10,ATGP4046-20,ATGP4046-50,ATGP4046-100,ATGP4046-250,ATGP4046-500,ATGP4046-1, ATGP3321

more info or order :

NKMAX product webpage