protein

Recombinant mouse IL-5 protein

ATGP4028

10 ug

140 USD

Recombinant mouse IL-5 protein

21-133aa

ATGP4028

NP_034688.1

IL5, also known as interleukin-5, is a member of the hematopoietin receptor superfamily and is comprised of a cytokine-specific alpha chain and the common beta chain that is shared among these cytokines for signaling. This interleukin produced by type-2 T helper cells and mast cells. It related hematopoietic cytokines that are important for allergic inflammation. It is a lineage-specific cytokine for eosinophilpoiesis and plays an important part in diseases associated with increased eosinophils, such as asthma. In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release. Recombinant mouse IL-5, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Interleukin-5, B-cell growth factor II, BCGF-II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, TRF, EDF

0.25mg/ml (determined by Absorbance at 280nm)

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Martinez-Moczygemba M., et al. (2003) J Allergy Clin Immunol. 112:653-665.;; Milburn MV., et al. (1993) Nature. 363:172-176.

> 90% by SDS - PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 3 ng/ml.

SDS-PAGE, Bioactivity

Mouse

Immunology

14.2kDa (122aa)

< 1 EU per 1ug of protein (determined by LAL method)

Mouse IL-5 in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 3 ng/ml.

MEIPMST VVKETLTQLS AHRALLTSNE TMRLPVPTHK NHQLCIGEIF QGLDILKNQT VRGGTVEMLF QNLSLIKKYI DRQKEKCGEE RRRTRQFLDY LQEFLGVMST EWAMEG

His-Tag

HEK293

Asp

Recombinant Protein

P04401

TRFB cell differentiation factor I, TRF, T-cell replacing factor, T cell replacing factor, Interleukin-5, Interleukin5, Interleukin 5, IL-5, IL5, IL 5, Eosinophil differentiation factor, EDF, Cytotoxic T-lymphocyte inducer, Cytotoxic T lymphocyte inducer, BCGF-II, BCGFII, BCGF II, B-cell growth factor II, B-cell differentiation factor I, B cell growth factor II, B cell differentiation factor I, ATGP4028-10ug, ATGP4028-20ug, ATGP4028-50ug, ATGP4028-100ug, ATGP4028-250ug, ATGP4028-500ug, ATGP4028-1mg, ATGP4028-010, ATGP4028-020, ATGP4028-050, ATGP4028-100, ATGP4028-250, ATGP4028-500, ATGP4028-01M