protein

Recombinant human PD-1 protein

ATGP4027

10 ug

72 USD

Recombinant human PD-1 protein

21-170aa

ATGP4027

NP_005009.2

PD-1, also known as programmed cell death protein 1, is a type 1 transmembrane glycoprotein, and is an immune-receptor belonging to the CD28/CTLA4 family. This protein is expressed on the surface of activated T-cell, B-cell, macrophages, myeloid cells and subset of thymocytes. It has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self-tolerance by suppressing T cell inflammatory activity. This prevents autoimmune diseases, but it can also prevent the immune system from killing cancer cells. This protein is an immune checkpoint and guards against autoimmunity through two mechanisms. It promotes apoptosis of antigen-specific T-cells in lymph nodes, And reduces apoptosis in regulatory T cells. PD-1 inhibitors, a new class of drugs that block PD-1, activate the immune system to attack tumors and are used to treat certain types of cancer. Blocking the PD-1 and PD-L1 interaction has proven successful in treating many different cancer type. Recombinant human PD-1, fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Programmed cell death protein 1, PDCD1, CD279, hPD-1, hPD-l, hSLE1, PD-1, PD1, SLEB2

0.5mg/ml (determined by absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Ishida Y., et al, (1992) EMBO J. 11:3887-3895.;; Lastwika KJ., et al, (2016) Cancer Res. 76:227-238.

> 95% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human PD-L1/B7-H1 (CAT# ATGP3631).

SDS-PAGE, Bioactivity

Human

Immunology, Apoptosis

42.9 kDa (383aa)

< 1 EU per 1ug of protein (determined by LAL method)

Human PDCD1 is coated at 5 ug/ml (100 ul/well) can bind Human PD-L1/B7-H1 (CAT# ATGP3631) in a Functional ELISA assay.

PGWFLDSPDR PWNPPTFSPA LLVVTEGDNA TFTCSFSNTS ESFVLNWYRM SPSNQTDKLA AFPEDRSQPG QDCRFRVTQL PNGRDFHMSV VRARRNDSGT YLCGAISLAP KAQIKESLRA ELRVTERRAE VPTAHPSPSP RPAGQFQTLV

hIgG-Tag

HEK293

Pro21

Recombinant Protein

Q15116

ATGP4027-10ug,ATGP4027-20ug,ATGP4027-50ug,ATGP4027-10ug,ATGP4027-250ug,ATGP4027-500ug,ATGP4027-1mg,ATGP4027-10,ATGP4027-20,ATGP4027-50,ATGP4027-100,ATGP4027-250,ATGP4027-500,ATGP4027-1,ATGP4027-10ug,ATGP4027-20ug,ATGP4027-50ug,ATGP4027-100ug,ATGP4027-250ug,ATGP4027-500ug,ATGP4027-1mg,ATGP4027-10,ATGP4027-20,ATGP4027-50,ATGP4027-100,ATGP4027-250,ATGP4027-500,ATGP4027-1