Recombinant human Noggin protein | NKMAX ATGP4020 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Noggin protein

catalog :

ATGP4020

quantity :

10 ug

price :

150 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Noggin protein

Domain :

28-232aa

Catalog number (SKU) :

ATGP4020

NCBI :

NP_005441.1

Description :

NOG, also known as noggin, is a secreted protein that is involved in the development of many body tissues, including nerve tissue, muscles, and bones and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP) -signaling pathway. It binds some BMPs such as BMP-4 with high affinity and others such as BMP-7 with lower affinity. This protein is an inhibitor of several bone morphogenetic proteins and cysteine-knot region of human Noggin are linked to multiple types of skeletal dysplasias that result in apical joint fusions. It also plays a key role in neural induction by inhibiting BMP4, along with other TGF-beta signaling inhibitors. Recombinant human Noggin, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names :

SYM1, SYNS1, SYNS1A, NOG, symphalangism 1, synostoses syndrome 1

Concentration :

0.25mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Zimmerman LB., et al, (1996) Cell. 86:599-606. ;; Groppe J., et al, (2002) Nature. 420:636-642.

Purity :

> 95% by SDS - PAGE

Bioactivity Comment Only :

Measured by ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells in the presence of 50ng/ml of human BMP-4. The ED50 range ≤ 0.5 ug/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Signal Transduction, Neuroscience

Molecular Weight :

23.8kDa (211aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human Nogging inhibit alkaline phosphatase production in the ATDC5 mouse chondrogenic cells in the presence of 50ng/ml of human BMP-4. The ED50 range ≤ 0.5 ug/ml.

Amino Acid Sequence :

QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC

Tag :

His-Tag

Expression System :

HEK293

Predicted nterm :

Gln

Product Type Name :

Recombinant Protein

UniProt No. :

Q13253

Internal Search Terms :

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more info or order :

NKMAX product webpage