Recombinant human Carbohydrate sulfotransferase 3/CHST3 protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Carbohydrate sulfotransferase 3/CHST3 protein

catalog :

ATGP4001

quantity :

10 ug

price :

160 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Carbohydrate sulfotransferase 3/CHST3 protein

Domain :

39-479aa

Catalog number (SKU) :

ATGP4001

NCBI :

NP_004264

Description :

CHST3, also known as chondroitin 6-O-sulfotransferase, is a member of sulfotransferase 1 family. The human CHST family is comprised of 14 enzymes and all members of this family are Golgi-localized type II membrane proteins. These enzymes utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. It can also sulfate Gal residues of keratan sulfate and Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. CHST3 Widely expressed in adult tissues. Expressed in heart, placenta, skeletal muscle and pancreas. Also expressed in various immune tissues such as spleen, lymph node, thymus and appendix. Recombinant human CHST3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

C6ST, C6ST-1, C6ST1HSD, Carbohydrate (chondroitin 6) sulfotransferase 3, Chondroitin 6-O-sulfotransferase 1, Chondroitin 6-sulfotransferase, CHST-3, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0, GST-0, HSD

Concentration :

0.25mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Hemmerich, S. and Rosen, S. Glycobiology 10:849-856.;; Uchimura, K. et al. J. Biol. Chem.277:1443-1450.;; Yusa, A. et al. J. Biol. Chem. 281:20393-20403.;;

Purity :

> 90% by SDS - PAGE

Bioactivity Comment Only :

Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that sulfate from PAPS to Chondroitin Sulfate per minute at pH 7.5, at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Signal Transduction, Cytoskeleton

Molecular Weight :

51.3kDa (450aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Amino Acid Sequence :

EKENKII SRVSDKLKQI PQALADANST DPALILAENA SLLSLSELDS AFSQLQSRLR NLSLQLGVEP AMEAAGEEEE EQRKEEEPPR PAVAGPRRHV LLMATTRTGS SFVGEFFNQQ GNIFYLFEPL WHIERTVSFE PGGANAAGSA LVYRDVLKQL FLCDLYVLEH FITPLPEDHL TQFMFRRGSS RSLCEDPVCT PFVKKVFEKY HCKNRRCGPL NVTLAAEACR RKEHMALKAV RIRQLEFLQP LAEDPRLDLR VIQLVRDPRA VLASRMVAFA GKYKTWKKWL DDEGQDGLRE EEVQRLRGNC ESIRLSAELG LRQPAWLRGR YMLVRYEDVA RGPLQKAREM YRFAGIPLTP QVEDWIQKNT QAAHDGSGIY STQKNSSEQF EKWRFSMPFK LAQVVQAACG PAMRLFGYKL ARDAAALTNR SVSLLEERGT FWVT

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ala

Product Type Name :

Recombinant Protein

UniProt No. :

Q7LGC8

Internal Search Terms :

C6ST, C6ST1, C6ST-1, C6ST1HSD, Carbohydrate (chondroitin 6) sulfotransferase 3, Carbohydrate sulfotransferase 3, Chondroitin 6 O sulfotransferase 1, Chondroitin 6 sulfotransferase, Chondroitin 6-O-sulfotransferase 1, Chondroitin 6-sulfotransferase, CHST 3, CHST3, CHST-3, Galactose/N-acetylglucosamine/N-acetylglucosamine 6 O sulfotransferase 0, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0, GST0, GST-0, HSD, ATGP4001-10ug, ATGP4001-20ug, ATGP4001-50ug, ATGP4001-100ug, ATGP4001-250ug, ATGP4001-500ug, ATGP4001-1mg, ATGP4001-010, ATGP4001-020, ATGP4001-050, ATGP4001-100, ATGP4001-250, ATGP4001-500, ATGP4001-01M

more info or order :

NKMAX product webpage