Recombinant mouse CHST5 protein | NKMAX ATGP3995 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse CHST5 protein

catalog :

ATGP3995

quantity :

10 ug

price :

160 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse CHST5 protein

Domain :

27-395aa

Catalog number (SKU) :

ATGP3995

NCBI :

NP_064334

Description :

CHST5, also known as Carbohydrate sulfotransferase 5, is a Golgi-embedded enzyme that is found in T cells, B cells and intestinal epithelium. This sulfotransferase utilizes 3-phospho-5-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. It also mediates sulfation of keratan in cornea. It acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N-acetyllactosamine structures. It may also have activity toward O-linked sugars of mucin-type acceptors. There is no CHST6 found in the mouse genome it is possible that mouse CHST5 plays a similar biological role to the human CHST6. Recombinant mouse CHST5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Chst5, Carbohydrate sulfotransferase 5, Galactose, N-acetylglucosamine, N-acetylglucosamine 6-O-sulfotransferase 4, GST4, Intestinal N-acetylglucosamine-6-O-sulfotransferase, I-GlcNAc6ST, Intestinal GlcNAc-6-sulfotransferase, mIGn6ST, N-acetylglucosamine 6-O-sulfotransferase 3, GlcNAc6ST-3, Gn6st-3, I-GlcNAc-6-ST, I-GlcN, GST-4. GST-

Concentration :

0.25mg/ml (determined by Bradford assay)

Formulation :

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Narentuya., et al, (2019) Sci Rep 9:4387.;; Yasutaka Hayashida., et al, (2006) Proc Natl Acad Sci U S A 103:13333-13338.

Purity :

> 90% by SDS - PAGE

Bioactivity Comment Only :

Specific activity is > 10,000 pmol/min/ug, and is defined as the amount of enzyme that sulfate from PAPS to N-acetyl-D-glucosamine per minute at pH 7.5, at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Mouse

Gene Category :

Immunology

Molecular Weight :

42.9kDa (380aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 10,000 pmol/min/ug, and is defined as the amount of enzyme that sulfate from PAPS to N-acetyl-D-glucosamine per minute at pH 7.5, at 37C.

Amino Acid Sequence :

SRQVP SSPAGLGERV HVLVLSSWRS GSSFVGQLFS QHPDVFYLME PAWHVWDTLS QGSAPALHMA VRDLIRSVFL CDMDVFDAYL PWRRNISDLF QWAVSRALCS PPVCEAFARG NISSEEVCKP LCATRPFGLA QEACSSYSHV VLKEVRFFNL QVLYPLLSDP ALNLRIVHLV RDPRAVLRSR EQTAKALARD NGIVLGTNGT WVEADPRLRV VNEVCRSHVR IAEAALHKPP PFLQDRYRLV RYEDLARDPL TVIRELYAFT GLGLTPQLQT WIHNITHGSG PGARREAFKT TSRDALSVSQ AWRHTLPFAK IRRVQELCGG ALQLLGYRSV HSELEQRDLS LDLLLPRGMD SFKWASSTEK QPES

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ala

Product Type Name :

Recombinant protein

UniProt No. :

Q9QUP4

Internal Search Terms :

,ATGP3995-10ug,ATGP3995-20ug,ATGP3995-50ug,ATGP3995-100ug,ATGP3995-250ug,ATGP3995-500ug,ATGP3995-1mg,ATGP3995-10,ATGP3995-20,ATGP3995-50,ATGP3995-100,ATGP3995-250,ATGP3995-500,ATGP3995-1

more info or order :

NKMAX product webpage