Recombinant human E-selectin/CD62E protein | NKMAX ATGP3994 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human E-selectin/CD62E protein

catalog :

ATGP3994

quantity :

10 ug

price :

76 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human E-selectin/CD62E protein

Domain :

22-556aa

Catalog number (SKU) :

ATGP3994

NCBI :

NP_000441

Description :

E-selectin, also known as CD62 antigen-like family member E (CD62E), is a member of the Selectin family. It is a selectin cell adhesion molecule expressed only on endothelial cells activated by cytokines. Like other selectins, it plays an important part in inflammation. The local release of cytokines IL-1 and TNF-α by Macrophages in the inflamed tissue induces the over-expression of E-selectin on endothelial cells of nearby blood vessels. As the inflammatory response progresses, chemokines released by injured tissue enter the blood vessels and activate the rolling leukocytes, which are now able to tightly bind to the endothelial surface and begin making their way into the tissue. While numerous pieces of in vitro and clinical evidence continue to support this hypothesis of E-selectin-mediated cancer metastasis, in vivo studies of cancer metastasis have shown that E-selectin knockout only minimally affects leukemic cell adhesion to bone immediately following injection. Recombinant human E-selectin, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

E-selectin, CD62E, ELAM, ELAM1, ELAM-1, ESEL, LECAM2, CD62 antigen-like family member E, endothelial-leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 2, SELE, CD62E antigen, ELAM1E-selectin, Endothelial leukocyte adhesion molecule 1

Concentration :

0.5mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Collins T, et al., (1991), J. Biol. Chem. 266:2466–2473.;; Kawase J, et al. (2009). Oncol Rep. 22:1293-1297.;;Matsumoto K, et al. (2010). Metabolism. 59:320-324.

Purity :

> 95% by SDS - PAGE

Bioactivity Comment Only :

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to human E-Seletin/CD62E coated plates 2ug/ml. This effect is more to 40%.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Immunology, Adhesion & Movement

Molecular Weight :

59.4kDa(541aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human E-Seletin/CD62E stimulates cell adhesion of the U937 human histiocytic lymphoma cells. This effect is more to 40%.

Amino Acid Sequence :

WSYNTSTEAM TYDEASAYCQ QRYTHLVAIQ NKEEIEYLNS ILSYSPSYYW IGIRKVNNVW VWVGTQKPLT EEAKNWAPGE PNNRQKDEDC VEIYIKREKD VGMWNDERCS KKKLALCYTA ACTNTSCSGH GECVETINNY TCKCDPGFSG LKCEQIVNCT ALESPEHGSL VCSHPLGNFS YNSSCSISCD RGYLPSSMET MQCMSSGEWS APIPACNVVE CDAVTNPANG FVECFQNPGS FPWNTTCTFD CEEGFELMGA QSLQCTSSGN WDNEKPTCKA VTCRAVRQPQ NGSVRCSHSP AGEFTFKSSC NFTCEEGFML QGPAQVECTT QGQWTQQIPV CEAFQCTALS NPERGYMNCL PSASGSFRYG SSCEFSCEQG FVLKGSKRLQ CGPTGEWDNE KPTCEAVRCD AVHQPPKGLV RCAHSPIGEF TYKSSCAFSC EEGFELHGST QLECTSQGQW TEEVPSCQVV KCSSLAVPGK INMSCSGEPV FGTVCKFACP EGWTLNGSAA RTCGATGHWS GLLPTCEAPT ESNIP

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Trp 22

Product Type Name :

Recombinant Protein

UniProt No. :

P16581

Internal Search Terms :

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more info or order :

NKMAX product webpage