protein

Recombinant human IL-4 protein

ATGP3983

10 ug

130 USD

Recombinant human IL-4 protein

25-153aa

ATGP3983

NP_000580

IL-4 is a multi-functional cytokine that plays a critical role in the regulation of immune responses. It induces differentiation of naive helper T cells to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. IL-4 is produced primarily by mast cells, Th2 cells, eosinophils and basophils. It is closely related and has functions similar to interleukin 13. This protein has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL-4 plays a dominant role in the development of allergic inflammation and asthma. Also, it is crucial in the development of allergic airway inflammation originates. Recombinant human IL-4, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Pitrakinra, MGC79402, Lymphocyte stimulatory factor 1, Interleukin-4 isoform 1, Interleukin 4, IL-4, BSF1, Binetrakin, BCGF1, B-cell stimulatory factor 1, B-cell growth factor 1, BCDF

0.5mg/ml (determined by Absorbance at 280nm)

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bhattarai P., et al, (2016) Cell Reports. 17: 941–948.;; Cheng Ho ., et al, (2016) Adv Exp Med Biol. 941:31-77.;; Marsha Wills-Karp., et al, (2008) Sci Signal. 1:pe55.

> 90% by SDS - PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 0.5 ng/ml.

SDS-PAGE, Bioactivity

Mouse

Immunology, Hormones & Cytokines

16.1kDa (139aa)

< 1 EU per 1ug of protein (determined by LAL method)

Human IL-4 in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 0.5 ng/ml.

HKCDIT LQEIIKTLNS LTEQKTLCTE LTVTDIFAAS KNTTEKETFC RAATVLRQFY SHHEKDTRCL GATAQQFHRH KQLIRFLKRL DRNLWGLAGL NSCPVKEANQ STLENFLERL KTIMREKYSK CSS

His-Tag

HEK293

Asp

Recombinant Protein

P05112

Pitrakinra, Pitrakinara, MGC79402, Lymphocyte stimulatory factor 1, Interleukin-4 isoform 1, Interleukin-4, Interleukin4, Interleukin 4 isoform 1, Interleukin 4, IL-4, IL4, IL 4, BSF-1, BSF1, BSF 1, Binetrakin, BCGF-1, BCGF1, BCGF 1, B-cell stimulatory factor 1, B-cell growth factor 1, BCDF, B cell stimulatory factor 1, B cell growth factor 1, ATGP3983-10ug, ATGP3983-20ug, ATGP3983-50ug, ATGP3983-100ug, ATGP3983-250ug, ATGP3983-500ug, ATGP3983-1mg, ATGP3983-010, ATGP3983-020, ATGP3983-050, ATGP3983-100, ATGP3983-250, ATGP3983-500, ATGP3983-01M