product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant canine IL-21 protein
catalog :
ATGP3974
quantity :
10 ug
price :
150 USD
more info or order :
product information
Product Type :
Proteins
Item Name :
Recombinant canine IL-21 protein
Domain :
18-146aa
Catalog number (SKU) :
ATGP3974
NCBI :
NP_001003347.1
Description :
IL-21, also known as Interleukin-21, is an important modulator of natural killer (NK) cell function. However, little is known about IL-21 function in canine NK cells because the phenotype of these cells remains undefined. NK cells proliferated rapidly in response to activation by IL-21 for 3 weeks, and IL-21 was able to induce changes in the mRNA expression of NK cell-related receptors and enhance the effector function of NK cells in perforin- and granzyme-B-dependent manners. The duration, frequency and timing of IL-21 stimulation during culture affected the rate of proliferation, patterns of receptor expression, cytokine production, and anti-tumor activity. It has a potential predicating that synergistic interactions of IL-21 with IL-2 and IL-15 play an important role in the proliferation, receptor expression, and effector function of canine NK cells. Recombinant canine IL-21, was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names :
Interleukin-21
Concentration :
0.5mg/ml (determined by absorbance at 280nm)
Formulation :
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
DJ Shin., et al. (2015) Vet Immunol Immunopathol. 15;165(1-2):22-33.;;Gui G., et al. (2017) Clin Immunol. 183:266-272.;;Parrish-Novak J., et al. (2000) Nature. 408(6808):57-63.
Purity :
> 95% by SDS-PAGE
Bioactivity Comment Only :
The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤ 2 ng/ml.
Applications :
SDS-PAGE, Bioactivity
Antigen Species :
Canine
Gene Category :
Immunology
Molecular Weight :
15 kDa (130aa), confirmed by MALDI-TOF
Endotoxin :
< 1 EU per 1ug of protein (determined by LAL method)
Bioactivity Comment and Pics :
Canine IL-21 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range ≤ 2 ng/ml.
Amino Acid Sequence :
MHKSSFQEQD LLLIRMRQLI DIVDQLKNYV NDLDPESLPA PEDVKRHCER SAFSCFQKVQ LKAANTGGNE QIINVLTKQL KRKLPPTNAG RRQKHRPACP SCDSYEKAPP KEFLERLKSL IQKMIHQHLS
Tag :
Non-Tagged
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
Q6L7I9
Internal Search Terms :
,ATGP3974-10ug,ATGP3974-20ug,ATGP3974-50ug,ATGP3974-100ug,ATGP3974-250ug,ATGP3974-500ug,ATGP3974-1mg,ATGP3974-10,ATGP3974-20,ATGP3974-50,ATGP3974-100,ATGP3974-250,ATGP3974-500,ATGP3974-1
more info or order :
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.