Recombinant human Thrombopoietin protein | NKMAX ATGP3970 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Thrombopoietin protein

catalog :

ATGP3970

quantity :

10 ug

price :

176 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Thrombopoietin protein

Domain :

22-353aa

Catalog number (SKU) :

ATGP3970

NCBI :

NP_000451.1

Description :

Thrombopoietin, also known as megakaryocyte growth and development factor (MGDF), is a member of EPO/TPO family. It is produced in the liver by both parenchymal cells and sinusoidal endothelial cells, as well as in the kidney by proximal convoluted tubule cells. It acts as a key regulator of megakaryocytopoiesis and thrombopoiesis that leads to platelet production. Also, this protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Thrombopoietin, like EPO, plays a role in brain development. It promotes apoptosis of newly generated neurons, an effect counteracted by EPO and neurotrophins. Recombinant human Thrombopoietin fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names :

THPO, MGDF, MKCSF, ML, MPLLG, THCYT1, TPO, thrombopoietin isoform 1 precursor, C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, ATGP3057

Concentration :

0.25mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Fishley B, et al, (2004) Growth Factors. 22:151-155.;; Chou FS, et al, (2011) J Cell Biochem. 112:1491-1498.;; Kong Z, et al, (2017) Blood. 130:1097-1103.;;

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range ≤10 ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Cardiovascular

Molecular Weight :

36.8kDa (343aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human Thrombopoietin stimulates cell proliferation of the MO7e human megakaryocytic leukemic cells. The ED50 range ≤10 ng/ml.

Amino Acid Sequence :

SPAPP ACDLRVLSKL LRDSHVLHSR LSQCPEVHPL PTPVLLPAVD FSLGEWKTQM EETKAQDILG AVTLLLEGVM AARGQLGPTC LSSLLGQLSG QVRLLLGALQ SLLGTQLPPQ GRTTAHKDPN AIFLSFQHLL RGKVRFLMLV GGSTLCVRRA PPTTAVPSRT SLVLTLNELP NRTSGLLETN FTASARTTGS GLLKWQQGFR AKIPGLLNQT SRSLDQIPGY LNRIHELLNG TRGLFPGPSR RTLGAPDISS GTSDTGSLPP NLQPGYSPSP THPPTGQYTL FPLPPTLPTP VVQLHPLLPD PSAPTPTPTS PLLNTSYTHS QNLSQEG

Tag :

His-Tag

Expression System :

HEK293

Predicted nterm :

Asp

Product Type Name :

Recombinant Protein

UniProt No. :

P40225

Internal Search Terms :

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more info or order :

NKMAX product webpage