protein

Recombinant human LILRB1/CD85j/ILT2 protein

ATGP3965

10 ug

120 USD

Recombinant human LILRB1/CD85j/ILT2 protein

24-461aa

ATGP3965

NP_006660.4

LILRB1, also known as leukocyte immunoglobulin like receptor B1, is a member of the leukocyte immunoglobulinlike receptor (LIR) family. The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. It is also expressed on the surface of B cells and monocytes, as well as subsets of NK cells, memory/effector CD8+ T cells, gamma δ T cells, and monocyte-derived dendritic cells. It binds to MHC-I as well as non-classical MHC-I molecules and the MHC-I mimetic UL18 encoded by cytomegalovirus. Recombinant human LILRB1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Leukocyte immunoglobulin-like receptor subfamily B member 1, LIR-1, LIR1, Leukocyte immunoglobulin-like receptor 1, CD85 antigen-like family member J, Immunoglobulin-like transcript 2, ILT-2, ILT2, Ig-like transcript 2, Monocyte/macrophage immunoglobulin-like receptor 7, MIR-7, MIR7, CD_antigen, CD85j, CD85 antigen-like family member J, PIRB, PIR-B, leucocyte Ig-like receptor B1, leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform, leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1, monocyte/macrophage immunoglobulin-like receptor 7, myeloid inhibitory receptor 7

0.25mg/ml (determined by absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Colonna, M. et al. (1997) J. Exp. Med. 186:1809-1818.;;Cosman, D. et al. (1997) Immunity 7:273-282.;;Ince, M.N. et al. (2004) Immunology 112:531-542.;;M Colonna et al. (1998) Immunology 7:3096-3100.

> 95% by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to LILRB1 coated plates 5ug/ml. This effect is more to 50%.

SDS-PAGE, Bioactivity

Human

Immunology

48.5kDa (446aa)

< 1 EU per 1ug of protein (determined by LAL method)

Human LILRB1 stimulates cell adhesion of the HSB2 human peripheral blood acute lymphoblastic leukemia cells. This effect is more to 50%.

GHLPKPTLWA EPGSVITQGS PVTLRCQGGQ ETQEYRLYRE KKTAPWITRI PQELVKKGQF PIPSITWEHT GRYRCYYGSD TAGRSESSDP LELVVTGAYI KPTLSAQPSP VVNSGGNVTL QCDSQVAFDG FILCKEGEDE HPQCLNSQPH ARGSSRAIFS VGPVSPSRRW WYRCYAYDSN SPYEWSLPSD LLELLVLGVS KKPSLSVQPG PIVAPEETLT LQCGSDAGYN RFVLYKDGER DFLQLAGAQP QAGLSQANFT LGPVSRSYGG QYRCYGAHNL SSEWSAPSDP LDILIAGQFY DRVSLSVQPG PTVASGENVT LLCQSQGWMQ TFLLTKEGAA DDPWRLRSTY QSQKYQAEFP MGPVTSAHAG TYRCYGSQSS KPYLLTHPSD PLELVVSGPS GGPSSPTTGP TSTSGPEDQP LTPTGSDPQS GLGRHLGV

His-Tag

Baculovirus

Gly24

Recombinant Protein

Q8NHL6

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